Introduction to digital PCR

Held in conjunction with Genomic Applications of qPCR, dPCR & NGS

25 May 2016, at 09:00-12:30 in Singapore

Introduction to digital PCR 

Description: 

This course gives an introduction to digital PCR and its current applications. We discuss the various strategies to measure dPCR including their advantages and limitations. Finally, we look into the future discussing forthcoming dPCR applications. 

• What is digital PCR?How does it work? 
• When is digital PCR useful? 
• How to use digital PCR for absolute quantification. 
• The advantage of digital PCR for rare mutation detection. 
• How to use digital PCR for analysis of Next generation sequencing libraries. 
• Gene expression analysis with digital PCR. 
• What is Poisson distribution? 
• Which controls are needed? 
• Commercially available instruments 
• Which are their differences (advantages/limitations)? 
• What to consider when designing assays for digital PCR. 
• Design and optimization of digital PCR experiments.