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3 for 2 Offer SELECTBIO Conferences Lab-on-a-Chip and Microfluidics Europe 2021Point-of-Care, Biosensors & Mobile Diagnostics Europe 20213D-Culture, Organoids & Organ-on-a-Chip Europe 2021The Space Summit 2021Flow Chemistry Summit 2021ePoster Award Prize

Introduction to Real-time qPCR


Held in conjunction with Genomics and Proteomics Research

26 Sep 2013, at 10.00am-5.00pm in Bangalore, India

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Course Description
Quantitative real-time PCR (qPCR) has emerged as the leading platform for nucleic acids analyses. It is becoming widely available making it possible to determine the amounts of specific nucleic acids and even proteins in biological samples with unsurpassed accuracy and sensitivity. This has opened new possibilities to detect pathogens in biological and environmental samples and to study and diagnose complex diseases. This 1-day course will give participants’ overview of q PCR technology and its possibilities, as well as insight in the future development of the technology platform and forthcoming applications.

Target Audience
This course is intended for researchers and laboratory personnel working with or planning to work with real-time PCR, researchers with interest in gene and protein expression, clinicians and pathologists with interest in molecular diagnostics, virologists, bacteriologists, and scientists with interest in food diagnostics and environmental testing.

Level of the Course
From beginners to intermediates

Course Outline (1 day course)
• Brief historic survey of PCR
• Basics of real-time PCR, applications and possibilities of the technology
• Present and forthcoming instrumentation platforms
• Probing technologies
• Multiplex qPCR
• Design and performance of qPCR experiments.
• Data pre-treatment and basic analysis
• Normalization strategies
• Quantification strategies
• Extraction of DNA and RNA
• Reverse transcription
• Experimental design and quality assessment
• Immuno qPCR for protein detection
• qPCR expression profiling
• Digital PCR
• Quality assessment and quality control
• The MIQE guideline

Mikael Kubista

Mikael Kubista, Professor/Founder, TATAA Biocenter AB


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