Best Laboratory Practices for the Flow Cytometry Laboratory

Jeffrey Louie, Consultant, FloCyte Associates Jeffrey Louie, MT (ASCP) is a clinical cytometrist with over 40 years experience in flow. He earned his BS at California State University at Los Angeles and is a California licensed Clinical Laboratory Scientist and ASCP registered with a speciality in Medical Technology. He was Chief Technologist in charge of all laboratory personnel and laboratory operations including research and development at the USC Flow Cytometry Laboratory, Los Angeles, CA for almost 20 years and before that, Medical Technologist Supervisor of Immunology/Serology Department at LAC/USC Medical Center, Los Angeles, CA for 9 years. Jeff consults for FloCyte in Clinical flow settings.
David Galbraith, Professor, University of Arizona David Galbraith obtained undergraduate and graduate degrees in Biochemistry from the University of Cambridge, and postdoctoral training as a NATO Fellow at Stanford University. His first academic appointment was at the University of Nebraska Lincoln, and he joined the University of Arizona in 1989 as Professor of Plant Sciences. His research has focused on the development of instrumentation and methods for the analysis of biological cells, organs, and systems. He pioneered the use of flow cytometry and sorting in plants, developing widely-used methods for the analysis of genome size and cell cycle status, and for the production of somatic hybrids. He also was amongst the first to develop methods for the analysis of gene expression within specific cell types, using markers based on Fluorescent Protein expression for flow sorting these cells, and microarray platforms for analysis of their transcriptional activities and protein complements. Current interests include applications of highly parallel platforms for transcript and protein profiling of minimal sample sizes, and for analysis of genetic and epigenetic mechanisms that regulate gene expression during normal development and in diseased states. He has published more than 170 scholarly research articles, holds several patents, and was elected a Fellow of the American Association for Advancement of Science in 2002.