A Microfluidic Immunoassay Format For the Determination of Cardiac Troponin T at the Point-of-Care
Eloisa Lopez-Calle,
Research & Development Line Manager & Development Lead,
Roche Diagnostics GmbH
A cardiac troponin T assay was developed in a microfluidic immunoassay format for the determination of the analyte with lab-like performance and meeting current clinical guidelines. The assay was completely run in a ready-to-use disposable with fully integrated reagents required for the analysis within less than 12 minutes. After sample application, 30µL of whole blood, the assay was automatically processed in the instrument, firstly by generating plasma via centrifugation, followed by incubation of a defined volume of the generated plasma with the immunoreagents. After washing, the fluorescence in the signal zone of the membrane was measured for the quantitation of cardiac troponin T.
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