Optofluidic Labs-on-Chip For Single Molecule Analysis
Holger Schmidt,
Narinder Kapany Professor of Electrical Engineering,
University of California-Santa Cruz
Lab-on-chip devices have long held the promise of providing a convenient
and rapid way to analyze small amounts of biological samples. However,
when pushed to the ultimate limit of single molecule sensitivity, the
detection mechanism is often based on off-chip elements. I will discuss a
chip-scale platform that offers both integrated optical and electrical
single molecule analysis. Optical integration is achieved by using
liquid-core waveguides interfaced with traditional photonic elements to
implement advanced functionalities. Examples include multiplex detection
of single viruses, simultaneous detection of proteins and nucleic acid
biomarkers, and front-to-back sample handling and single DNA detection
on a single chip. Electrical single molecule analysis is achieved by
integration of solid-state nanopores. Novel nanopore detection
capabilities such as feedback-controlled delivery of single molecules to
a fluidic channel are demonstrated. The combination of both optical and
electrical detection modalities results in a novel, high throughput
platform for single molecule analysis.
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