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SELECTBIO Conferences Lab-on-a-Chip and Microfluidics World Congress 2022

Lab-on-a-Chip and Microfluidics World Congress 2022 Agenda


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Monday, 12 December 2022

00:00

Gregory NordinKeynote Presentation

Title to be Confirmed.
Gregory Nordin, Professor, Brigham Young University, United States of America

00:00

Nicole PammeKeynote Presentation

Title to be Confirmed.
Nicole Pamme, Professor in Analytical Chemistry, Stockholm University, Sweden

00:00

Lydia SohnKeynote Presentation

Title to be Confirmed.
Lydia Sohn, Almy C. Maynard and Agnes Offield Maynard Chair in Mechanical Engineering, University of California-Berkeley, United States of America

00:00

Nancy AllbrittonPlenary Presentation

Picoliter Thin Layer Chromatography (pTLC) for Assay of Lipid Signaling in Single Cells
Nancy Allbritton, Frank and Julie Jungers Dean of the College of Engineering and Professor of Bioengineering, University of Washington in Seattle, United States of America

Lipid signaling pathways regulate a multitude of cellular behaviors in humans including cell proliferation, death, migration, and many other attributes. These pathways are implicated in a host of diseases including cancer and autoimmune pathologies and as such a large number of compounds are now in clinical trials to target the critical enzymes within these signaling cascades. However, most existing technologies for assay of cellular lipids possess poor sensitivity and require large sample volumes making them unsuitable for the assay of mammalian cells which are 1 pL in volume and may possess as little as 10-20 moles of key regulatory lipids. To address this challenge, an array of open microchannels filled with a monolithic silica was developed using microfabrication and sol-gel chemistry. When ultra-small volumes (<1 nL) of fluorescent lipids including [phosphatidylinositol 4,5-bisphosphate, phosphatidylinositol 3,4,5-trisphosphate, phosphatidylcholine (PC), phosphatidylethanolamine (PE), 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine, sphingosine, sphingosine-1-phosphate (S1P), and ceramide] were spotted at the channel inlet followed by separation, the lipids were detectable with excellent sensitivity (10-20 moles). To demonstrate the power of pTLC, leukemic cells were loaded with fluorescent PC, PE or sphingosine and a single cell printed at the inlet of a pTLC channel. PE and PC within a single cell were separated by pTLC in less than 1 min with excellent resolution and detection limits. When cells were loaded with a fluorescent sphingosine, the conversion of sphingosine to S1P was also detected and the reaction blocked by sphingosine kinase inhibitors. These novel biomedical microdevices can be fabricated in large scale arrays for fully automated, high-throughput assay of ultra-small-sized samples as well as for screening of therapeutic modulators of lipid signaling. Additionally pTLC enables assay of the diversity of signaling activity of key lipid pathways amongst single cells. These and other advances in biomedical microdevices are paving the way for rapid discoveries in basic and pharmaceutical sciences, as well as personalized medicine.

00:00

Leanna LevineConference Chair

Welcome and Introduction by Conference Chairperson and an Overview of the Field of Microfluidics
Leanna Levine, Founder & CEO, ALine, Inc., United States of America

00:00

Steve SoperPlenary Presentation

Title to be Confirmed.
Steve Soper, Foundation Distinguished Professor, Director, Center of BioModular Multi-scale System for Precision Medicine, The University of Kansas, Adjunct Professor, Ulsan National Institute of Science & Technology, United States of America

00:00

Noah MalmstadtKeynote Presentation

Title to be Confirmed.
Noah Malmstadt, Professor, Mork Family Dept. of Chemical Engineering & Materials Science, University of Southern California, United States of America

00:00

Holger SchmidtKeynote Presentation

Title to be Confirmed.
Holger Schmidt, Narinder Kapany Professor of Electrical Engineering, University of California-Santa Cruz, United States of America

00:00

Mehmet TonerPlenary Presentation

Title to be Confirmed.
Mehmet Toner, Helen Andrus Benedict Professor of Biomedical Engineering, Massachusetts General Hospital (MGH), Harvard Medical School, and Harvard-MIT Division of Health Sciences and Technology, United States of America

00:00

John WikswoPlenary Presentation

The Convergence of Microfluidics, Artificial Intelligence, and Machine Learning
John Wikswo, Gordon A. Cain University Professor, A.B. Learned Professor of Living State Physics; Founding Director, Vanderbilt Institute for Integrative Biosystems, Vanderbilt University, United States of America

Wednesday, 14 December 2022

00:00

Dino Di CarloKeynote Presentation

Title to be Confirmed.
Dino Di Carlo, Professor and Vice Chair of Bioengineering, University of California-Los Angeles, United States of America


Agenda is not currently available
Add to Calendar ▼2022-12-12 00:00:002022-12-14 00:00:00Europe/LondonLab-on-a-Chip and Microfluidics World Congress 2022Lab-on-a-Chip and Microfluidics World Congress 2022 in Long Beach, CaliforniaLong Beach, CaliforniaSELECTBIOenquiries@selectbiosciences.com