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SELECTBIO Conferences Point-of-Care, Biosensors and Rapid Dx Europe 2024

Point-of-Care, Biosensors and Rapid Dx Europe 2024 Agenda

Co-Located Conference Agendas

Circulating Biomarkers and Extracellular Vesicles Europe 2024 | Lab-on-a-Chip and Microfluidics Europe 2024 | Organoids and Spheroids Europe 2024 | Point-of-Care, Biosensors and Rapid Dx Europe 2024 | 

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Monday, 24 June 2024


Please View Conference Programming Details in the:

LOACEU2024 Track Morning Plenary Session -- Coolsingel Room

ORGANOIDS Track Afternoon Session -- Coolsingel Room

EV Track Afternoon Session -- Conrad Room

Tuesday, 25 June 2024


Please View Conference Morning Programming Details in the:

LOACEU2024 -- Coolsingel Room

Organoids & Spheroids -- Conrad Room


Networking Buffet Lunch -- Network with Exhibitors and View Posters

Session Title: Emerging Technologies and Themes in the Point-of-Care and Rapid Diagnostics Field

Session Venue: Coolsingel Room

Session Chairperson: Professor Dr. Nicole Pamme


Nicole PammeKeynote Presentation

Microfluidic Devices for On-Site Chemical Sensing
Nicole Pamme, Professor in Analytical Chemistry, Stockholm University, Sweden

Our research centers on the study of microfluidic lab-on-a-chip devices applied to environmental analysis, biomedical research and the synthesis of smart materials. Microfluidic devices offer the possibility for in-the-field and point-of-care analysis provided the devices are portable, require only minimal external instrumentation and little power and are robust. In our group, we are investigating a developing paper microfluidic devices for on-site measurement of water quality markers that are being tested by members of the general public who upload data via a custom-built app for data collection with high spatial and temporal resolution. Furthermore, we develop on-site workflows that enable farmers in resource-limited settings to monitor soil chemistry on their own land.


Lucia CiglarKeynote Presentation

Multi-Omics and EV-based Biomarkers For Complex Disease Diagnostics From Blood, Saliva or Cerebrospinal Fluid
Lucia Ciglar, Scientist, AIT Austrian Institute of Technology (AIT), Austria

The discovery and validation of circulating biomarkers for minimally invasive diagnostics of complex diseases have been a major focus of AIT’s Molecular Diagnostics group. Multi-omics approaches for biomarker development from both tissue and cell-free body fluids, including the utilization of EV-derived miRNAs, have been successfully established. In this presentation, the extensive multi-omics technology workflows developed by our research group will be introduced and selected results from a study conducted on patients with coronary artery disease will be highlighted, demonstrating the power of integrating various omics layers to identify and validate circulating biomarkers. Additionally, diverse EV biomarker studies will be reported, showcasing research across a range of diseases, including metabolic, immune-mediated, and cardiovascular diseases. EVs derived from different biofluids, such as serum, plasma, cell-free saliva, and cerebrospinal fluid, have been utilized, demonstrating the versatility and promise of EVs in disease diagnostics.


Ines AntunesKeynote Presentation

ESA SciSpacE Human Research Programme
Ines Antunes, Science Coordinator, European Space Agency (ESA), Netherlands

Presentation of the challenges faced by astronauts during long-duration space missions and how the European Space Agency (ESA) is supporting their health and performance through research on the impact of space conditions on the human body and the development of countermeasures and point of care technologies to investigate changes and mitigate those consequences. The presentation will focus on ESA's SciSpacE team, which conducts research in ground analogues, suborbital platforms, and on the International Space Station (ISS), and how the research conducted in space can benefit terrestrial medicine. The process of ideation, call for proposals, review, selection, and implementation of research projects will also be outlined. The presentation aims to provide insights into how ESA is working to keep astronauts healthy and productive during long-term space missions and how these efforts can also benefit medical science on Earth.


Mid-Afternoon Coffee Break and Networking


Best Poster Awards Sponsored by Lab-on-a-Chip Journal, Royal Society of Chemistry (RSC) -- In the Coolsingel Room


Tackling the Global Health Threat Posed by Fungal and Oomycete Diseases Using Lab-on-a-Chip Devices
Ayelen Tayagui, Research Fellow, University of Canterbury, New Zealand

Lab-on-a-Chip devices can enhance the study of disease-causing microorganisms and tackle their effects on global health.  We describe the design, manufacture and use of devices use to study the forces these organisms use to to infect their hosts.


Towards Continuous Cytokine Monitoring in Organ-based Platforms
Maud Linssen, Researcher, Eindhoven University of Technology (TU/E), Netherlands

Investigations on organ-based platforms such as organoids, organ-on-a-chip, and transplantation organs, require monitoring strategies in order to optimally control the biological systems. However, there is a lack of tools to continuously measure specific low-concentration biomarkers with minimal perturbation. Biosensing by Particle Motion (BPM) is a sensing method with single-molecule resolution that has been specifically designed to enable the continuous monitoring of biomolecules at low concentrations, such as nucleic acids, metabolites, proteins and hormones. The method relies on tracking the motion of individual biofunctionalized particles (1 µm in diameter) that interact with a biofunctionalized substrate. The particles switch between bound and unbound states due to reversible single-molecule interactions, dependent on the presence of analyte molecules. Recently, sampling by microdialysis has been investigated [3]. In this paper we present the development of a BPM sensor to measure cytokines, exemplified with the detection of Interleukin-6 and TNF-a. We will present a study of molecular binders and coupling strategies, focusing on sensitivity and reversibility of the sensor. Furthermore, measurements with microdialysis will be shown. Finally, we will discuss the prospects of using BPM and microdialysis for the continuous monitoring of low-concentration biomarkers in organ-based platforms.


Luxembourg Institute of Science and TechnologyElectroMed – A Programmable Peptide/Protein Screening Platform for Immunology
Cesar Pascual García, Lead Research Scientist, Luxembourg Institute of Science and Technology

Immunology has advanced significantly in recent years thanks to a better knowledge of molecular protein recognition, which has allowed for the creation of innovative treatment options such as genetic vaccines and cell therapies. However, there is a need to accelerate the process of epitope recognition by proteins in order to generate personalized therapies. ElectroMed has been a project with six European partners in which we created a concept for a programmable peptide screening platform based on an array of Immuno-FET sensors with programmable peptide receptors synthesized in-situ using electrochemically generated acid.

A proof-of-concept has been demonstrated by controlling the deprotection of acid labile groups, which enables a high purity synthesis of crude peptides. We remove diffusion-limited processes in chemistry by automating the peptide synthesis and using microfluidic flow in-situ synthesis in planar sensors, allowing us to expedite time to results. FET sensors have the potential to provide online quality control of peptide synthesis by detecting the fingerprints of acid labile group protection and deprotection, as well as superior information quality because label-free sensors can provide kinetic information.

In conclusion, our approach has the potential to speed research into peptide protein screening of linear peptide sequences. In this talk we will review our results and provide the context for the applications in immunology.


Parallel High-Throughput Single-Cell Printing Platform for Optoporation-Mediated Large Cargo Delivery
Gayathri R, PhD Scholar (Student), Indian Institute of Technology (IIT)-Madras, India

Effective intracellular delivery of biomolecules is crucial in analysing and engineering cell functions, for applications in therapeutic development, diagnostics, and drug delivery towards personalised medicine. Traditional bulk cell culture models employed so far for intracellular delivery, overlook cell population heterogeneity, while single-cell patterning offers robust statistical analyses without compromising cellular variability. In this work, a high-throughput single-cell patterning approach using microcontact printing is employed, where a micro-pillar stamp is used to imprint distinct proteins and finally pattern single-cell to small clusters of cells depending on the micro-pillar diameters. This approach is universal for any protein-cell combination and has 97-99 % patterning efficiency. This single-cell patterning was used as a platform for massively parallel optoporation-mediated intracellular delivery of small to very large biomolecules such as PI dye (668 Da), Dextran (3000 MW), SiRNA (20-24 bp), and enzyme (464 kDa). An easy-to-fabricate and simple-to-use 2D array of titanium micro-dish device is used to facilitate near infrared mediated optoporation. which disrupts the cell plasma membrane, allowing biomolecules to enter into cells with high efficiency (~96±2%) and cell viability (~98±1%). Our compact, robust, and simple-to-print approach has potential for single-cell therapy and diagnostics research, providing high specificity and subcellular accuracy in single-cell level biological processes.


Temporal Influence of Extracellular Vesicle-Depleted Serum on Extracellular Vesicles and Endothelial Cells
Luiz Fernando Cardoso Garcia, Scientist, Biociências e Biotecnologia do Instituto Carlos Chagas, Brazil

Extracellular vesicles (EVs) are generated by various cell types, including endothelial cells. The presence of EVs in fetal bovine serum (FBS), commonly used in cell culture, has been recognized as a potential confounding factor. To elucidate this, human brain microvascular endothelial cells (HBMEC) were cultured for 2 or 24 hours in the presence of EV-depleted FBS (EVdS). Cell viability, gene and protein expression, and EVs isolated from these cells were assessed. Additionally, EV uptake, ICAM-1 expression, and monocyte adhesion to HBMEC exposed to EVs were also examined. Elevated apoptosis rates in cells cultured with EVdS for 2 and 24 hours was observed. Within 2 hours, there was an upregulation of IL8, followed by the downregulation of IL6 and IL8 after 24 hours. Proteomic analysis revealed that EVs cultured for 2 hours (EV2h) were enriched in proteins associated with ribosomes and carbon metabolism, while those cultured for 24 hours (EV24h) exhibited proteins linked to cell adhesion and platelet activation. Moreover, HBMECs exposed to EV2h displayed increased ICAM-1 expression and monocyte adhesion compared to cells exposed to EV24h. These findings highlight that HBMECs cultured with EVdS produce EVs with distinct physical characteristics and protein content that varies across time.


Optical Bunching of Crystals in a 3D Printed Microfluidic Device for Enhancing Sample Delivery in Serial Crystallography
Anusha Keloth, Postdoctoral Research Fellow, Deutsches Elektronen-Synchrotron DESY, Germany

This paper presents the optical bunching of protein microcrystals in a 3D printed microfluidic device for improving the sample delivery for serial crystallography studies at X-ray free electron lasers.


Close of Day 2 Conference Programming

Add to Calendar ▼2024-06-24 00:00:002024-06-25 00:00:00Europe/LondonPoint-of-Care, Biosensors and Rapid Dx Europe 2024Point-of-Care, Biosensors and Rapid Dx Europe 2024 in Rotterdam, The NetherlandsRotterdam, The