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SELECTBIO Conferences Advances in qPCR and dPCR

Advances in qPCR and dPCR Agenda



Co-Located Conference Agendas

Advances in Cellular Assays & Cell Culture | High Content Analysis | Advances in Automation & Robotics | Advances in NGS & Big Data | Advances in qPCR and dPCR | 

Print Agenda

Wednesday, 14 May 2014

08:00

Registration


Single Cell Analysis

09:00

Mikael KubistaKeynote Presentation

High Throughput Single Cell and Subcellular Expression Profiling
Mikael Kubista, Professor/Founder, TATAA Biocenter AB, Sweden

Biological samples are composed of large number of cells of different types. When studying traditional samples containing many cells only the collective response of all the cells present is measured. However, the cells may respond differently and a small subpopulation may be critical. Today, these systems can be studied using single cell expression profiling. Here we apply single cell profiling to study the response of astrocytes to brain trauma using mouse model. We also study asymmetric cell division during early development of Xenopus laevis by single cell and intracellular profiling using qPCR tomography.

10:00

Coffee & Networking in Exhibition Hall

10:45

In-vivo Single Cell Transcript Analyses for Systems Modelling
Philip Day, Reader, The University of Manchester, United Kingdom

Single cell measurements of DNA, mRNA and protein for bcr-abl was measured in K562 cells to produce a steady state model of BCR.ABL protein abundance per mRNA molecule. Impact of heterogeneity and development of in-vivo transcript measurements will be discussed.

11:30

Advanced Molecular Tools for Proteome Analyses
Masood Kamali-Moghaddam, Associated Professor, Uppsala University, Sweden

This presentation will illustrate a set of molecular tools with very high sensitivity and specificity for detection and localization of proteins and their interactions. Furthermore, the application of the methods in the search for biomarkers will be discussed.

12:15

Lunch & Networking in Exhibition Hall

13:30

Poster Viewing Session


Standardisation & Validation

14:15

Evidence Based Guidelines for the Pre-analytical Phase of RNA Testing in Blood Samples
Francesca Malentacchi, Postdoctoral Fellow, University Of Florence, Italy

Within the EC funded project SPIDIA (www.spidia.eu), pan-European external quality assessment (EQA) programmes were implemented for blood RNA based tests. The results demonstrated that the use of blood collection tubes containing cellular RNA stabilizers allows a reliable gene expression analysis whereas RNA measurements deriving from EDTA blood collection tubes showed an ex-vivo gene-dependent induction even 24h after blood collection. The results of the SPIDIA RNA EQAs studies have been used for the development of a Technical Specification by the European Committee for standardization (CEN).

15:00

qPCR in the Eyes of Europe’s No. 1. Commercial Service Supplier; Challenges and Innovative Solutions
Jens Bjorkman, CSO Manager, TATAA Biocenter AB, Sweden

Since the publication of the MIQE (Minimum Information for Publication of Quantitative Real-Time PCR Experiments) guidelines in 2009 users’ awareness and clients’ requests of quality control (QC) in qPCR has increased. I will describe some of our research into this field and provide real-life examples on how quality assurance protocols and strategies in qPCR workflow improve the performance and reliability of results.

15:45

Coffee & Networking in Exhibition Hall


Mutation Analysis

16:30

Determination and Follow-up of a Mutational Profile with Circulating Tumour DNA Analysis
Florent Mouliere, Researcher, Nitzan Rosenfeld Group, University of Cambridge, United Kingdom

Circulating tumour DNA (ctDNA) exhibits the same genetic alterations from its tumour of origin, and can be used as a “liquid biopsy” for monitoring tumour progression. Q-PCR, digital PCR and next-generation sequencing are technologies of choice for determining and following-up a mutational profile with ctDNA.

17:15

Selective Amplification of Rare Mutant Sequences in Samples Containing Abundant Wild-type Sequences
Fred Kramer, Program Director, University of Medicine and Dentistry of New Jersey, United States of America

SuperSelective primers, by virtue of their unique design, employ thermodynamic and kinetic principles that enable the routine quantitation of as few as 10 mutant molecules in samples containing 1,000,000 wild-type molecules, even if the mutation is only a single-nucleotide polymorphism.

18:15

End of Day One

Thursday, 15 May 2014


New Developments in PCR

08:15

Carl WittwerKeynote Presentation

Extreme PCR: Robust, Efficient Amplification in < Min
Carl Wittwer, Professor, University of Utah, United States of America

PCR combines higher primer and polymerase concentrations with cycle times of less than 2 s, resulting in specific, high-yield amplification as demonstrated by gels, melting curves and real time analysis. Monovalent cations and dsDNA dyes inhibit, while DMSO enhances, polymerase extension.

09:15

Finding Needles in a Haystack with Q-PCR – Rare Mutations, Single Cell Genotyping and DNA Methylation Analysis
Jorg Tost, Director, Centre National de Genotypage CEA, France

E-ice-COLD PCR enables reliable detection and sequence based identification of low prevalence mutations with clinical relevance in multiple types of patient samples including plasma. In addition, a simple protocol using standard real-time thermocyclers allows analyzing genotypes and DNA methylation patterns in cell-sorted single cells.

10:00

Coffee & Networking in Exhibition Hall


Clinical Applications of Digital PCR

10:45

Digital PCR Goes COLD: Application of COLD-PCR in Digital Format Enables Quantitative and Multiplexed Mutation Scanning
Elena Castellanos-Rizaldos, Instructor in Radiation Oncology, Dana-Farber Cancer Institute/Harvard Medical School, United States of America

As currently applied, digital PCR can only be used to detect known mutations at single sequence positions. By merging COLD-PCR and digital -PCR technologies we enable digital mutation scanning, a new frontier for digital-PCR. A single reaction can now interrogate numerous mutations, and replaces multiple individual digital-PCR reactions.

12:15

Lunch & Networking in Exhibition Hall

13:30

Poster Viewing Session

14:15

Digital PCR in the Clinical Virology Laboratory - Applications and Limitations
Keith Jerome, Professor, University of Washington, United States of America

Digital PCR (dPCR) allows sensitive and accurate absolute quantitation of nucleic acid samples without the need for a standard curve. While dPCR is still mainly used in research settings, the characteristics of dPCR also make it attractive in the clinical laboratory. In this talk we will discuss applications of dPCR in the diagnostic virology laboratory, including ultraprecise cytomegalovirus monitoring, diagnosis of chromosomally integrated human herpesvirus 6, and quantitation of latent HIV reservoirs.

15:00

Quantification of Bacterial DNA for Metagenomic Control Materials
Denise O'Sullivan, Researcher, LGC Genomics, United Kingdom

In this study we use digital PCR to quantify the component organisms of a metagenomic control material comprising 10 common human pathogens mixed together in different proportions.

15:45

Coffee & Networking in Exhibition Hall

16:15

qPCR as a Tool for Early Detection of Alzheimer´s Disease
Petar Podlesniy, Postdoctoral Research Associate, Consejo Superior De Investigaciones Científicas, Spain

I will share our experience in the implementation of ddPCR for quantitation of low abundance targets (in the range of hundredths molecules per sample) as the circulating nucleic acids in biological fluids. Special emphasis will be made on the sample preparation and the controls used.

17:00

Analysing Circulating Tumor DNA in Clinical Trials – Today and in Future
Arndt Schmitz, Head/Senior Scientist, Bayer Schering Pharma AG, Germany

BAYER experiences with analysing 2,000 samples in our clinical trials with the most sensitive technique available will be reported. The field will soon be more complex, with established techniques becoming companion diagnostics and new technologies such as NGS entering.

17:45

Close of Conference


Add to Calendar ▼2014-05-14 00:00:002014-05-15 00:00:00Europe/LondonAdvances in qPCR and dPCRAdvances in qPCR and dPCR in Barcelona, SpainBarcelona, SpainSELECTBIOenquiries@selectbiosciences.com