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SELECTBIO Conferences Lab-on-a-Chip and Microfluidics Europe 2022

Lab-on-a-Chip and Microfluidics Europe 2022 Agenda



Co-Located Conference Agendas

Point-of-Care, Biosensors and Mobile Diagnostics Europe 2022 | Organoids and Organs-on-Chips Europe 2022 | Lab-on-a-Chip and Microfluidics Europe 2022 | 

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Tuesday, 21 June 2022

08:00

Conference Registration, Materials Pick-Up, Morning Coffee and Tea


Session Title: Lab-on-a-Chip and Microfluidics 2022

09:00

Claudia GärtnerConference Chair

A Microfluidic Handheld Platform - Flexible Use With and Without Instrument
Claudia Gärtner, CEO, microfluidic ChipShop GmbH, Germany

09:30

Valérie TalyKeynote Presentation

Microfluidics For Cancer Research
Valérie Taly, CNRS Research Director, Group leader Translational Research and Microfluidics, University Paris Descartes, France

Droplet-based microfluidics has led to the development of highly powerful tools with great potential in High-Throughput Screening where individual assays are compartmentalized within aqueous droplets acting as independent microreactors. Thanks to the combination of a decrease of assay volume and an increase of throughput, this technology goes beyond the capacities of conventional screening systems. Added to the flexibility and versatility of platform designs, such progresses in the manipulation of sub-nanoliter droplets has allowed to dramatically increase experimental level of control and precision. This technology has been key to recent developments in cancer research. These recent developments will be central to this presentation. In particular, we will show how by combining microfluidic systems and clinical advances in molecular diagnostic we have developed an original method to perform millions of single molecule PCR in parallel to detect and quantify a minority of target sequences in complex mixture of DNA with a sensitivity unreachable by conventional procedures. To demonstrate the pertinence of our procedures to overcome clinical oncology challenges, the results of clinical studies will be presented.

10:00

Holger SchmidtKeynote Presentation

Ultra-Sensitive Optofluidic Platform for Point-of-Care Molecular Diagnostics
Holger Schmidt, Narinder Kapany Professor of Electrical Engineering, University of California-Santa Cruz, United States of America

Lab-on-chip systems can form the basis for next-generation molecular biosensors for point-of-care use. In order to fulfill this potential, they need to combine excellent specificity and sensitivity with speed, robustness, and low cost. I will describe a waveguide-based optofluidic platform that is ideal for this purpose. Specifically, I will review ultrasensitive performance using the example of  fluorescence and label-free detection of SARS-CoV-2 from clinical nasal swab samples with single molecule sensitivity. Multiplex detection of single viral antigens is demonstrated along with dual detection of viral DNA and antigen as well as label-free nanopore sensing of single RNAs with 2,000x enhanced detection rate. In addition, I will discuss approaches geared at adapting this platform for point-of-case use, including novel signal analysis algorithms and implementation of real-time analysis of single-molecule fluorescence signals.

10:30

Mid-Morning Coffee Break and Networking in the Exhibit Hall

11:00

IMT MicrotechnologiesWhere Photonics Meet Microfluidics – Challenges and Opportunities For Consumables For Life Sciences and Diagnostics Applications in Glass
Tobias Bauert, Business Development Manager, IMT Microtechnologies

Fluid manipulation on the micro-scale allows new applications in various fields including life sciences and diagnostics. Many of these applications incorporate bio-sensors in a microfluidic setup. The choice of transduction mechanism for these bio-sensors creates the boundary conditions not only for the bio-chip itself but for the surface and bulk materials as well as the manufacturing technologies. Glass sometimes is the only choice due to limiting optical specifications or very harsh operating conditions. By harvesting the power of CMOS manufacturing technologies with non-CMOS compatible materials on glass, cost effective consumables for life sciences become reality in all volumes from prototyping to large scale manufacturing.

11:30

EmulseoEmulseo: Formulation Solutions For Microfluidic Applications
Ariane Peyret, Technical Support and Marketing Manager, Emulseo

Water-in-oil emulsion droplets produced with microfluidic devices have become widely used as powerful tools for biological and chemical applications including screening, material synthesis, single-cell analysis, digital polymerase chain reaction assays (dPCR) or in vitro diagnostics. Droplet microfluidics enable chemical and biological experiments to be performed at high-throughput in a confined and controlled environment with enhanced efficiency and using minimal amount of starting material. These applications all require reliable formulation tools that meet essential quality criteria. Especially, surfactants are key to generating stable emulsions. Being able to fully control their synthesis in a reproducible way and at large scales is crucial. For these reasons, Emulseo has worked on developing custom-made solutions and ready-to-use formulations such as fluorinated surfactants, fluorinated oils, surface treatments and other specialty chemicals.

12:00

STRATEC Consumables GmbHMoving Diagnosis Closer to the Patient: Smart Consumables For Novel Point-of-Care Solutions
Iris Prinz, Head of Sales and Business Development, STRATEC Consumables GmbH

Within the presentation we will focus on a Point-of-Care solution realized at STRATEC Consumables jointly with Femtonics. This Point-of-Care cartridge includes several challenges e.g. manufacturing of fluidics including opening mechanism for blisters by injection molding, content loading like dispensing and lyophilization as well as several assembly steps in order to realize a diagnostic platform with the aim to detect respiratory diseases.

12:30

Andrew J deMelloKeynote Presentation

High-Throughput Microfluidic Cytometry
Andrew J deMello, Professor of Biochemical Engineering & Institute Chair, ETH Zürich, Switzerland

Flow cytometry is a ubiquitous analytical technique for enumerating and sorting cellular populations. Because of its quantitative and multi-parametric nature and the fact that cells may be probed at high throughputs, flow cytometry is considered to be the gold standard method for measuring the physical and chemical properties of a large cellular populations. Although modern-day flow cytometers are adept at processing thousands of cells per second, almost all require unacceptably large sample/reagent volumes and do not allow the acquisition of spatially resolved information. To address these issues, imaging flow cytometry (IFC) marries the advantages of optical microscopy and flow cytometry to allow the for high-throughput imaging of cells within flowing environments. I will describe how we have leveraged the capabilities of microfluidic systems to form novel IFC platforms able to manipulate, process and assay cells in a controlled and high-throughput manner. Examples will include chip-based imaging flow cytometers that leverage either inertial or viscoelastic focusing. Such systems operate at throughputs exceeding 400,000 cells/s, extract fluorescence, brightfield, and darkfield images and are capable of multi-parametric quantification and sub-cellular localization of structures down to 500 nm. Finally, I will describe recent activities focused on the development of a viscoelastic-based microfluidic platform able to measure the mechanical properties of cells at rates of up to 100,000 cells per second. Here, fluid elasticity is used to focus and deform cells in an integrated manner and without the need for a sheath fluid.

13:00

Networking Lunch in the Exhibit Hall with Exhibitors and Conference Sponsors + Poster Viewing


Session Title: Convergence of Microfluidics and POC Diagnostics Fields

14:30

Micronit BVMicrofluidics Applications in the Life Sciences Sector
Chandini Kadian, Business Development and Strategy, Micronit BV

Microfluidics is the science of manipulating small volumes of liquids. This field is rapidly evolving and can have an enormous impact on the progress in science and healthcare. This usually is executed on microchips made with manufacturing techniques also used in the semiconductor industry, like lithography, etching and thin film deposition. The chips contain small channels, which enable accurate control of liquids and chemical reactions. The use of small volumes allows quicker temperature shifts and faster liquid displacement. Moreover, microfluidics enables the automation and integration of complex operations on-chip, with reduced sample and expensive reagent volumes. With such properties, microfluidics can fit applications in the healthcare sector along with other microtechnologies. The approach starts from a conceptual design to prototyping to a final design and process transfer for volume manufacturing. A flexible approach allows the choice of a variety of materials and hybrid material combinations and customized designs e.g. for successful integration of multiple functions. This approach is of particular interest for developing relative complex designs with multiple micro- and nanostructures and functionalities on board. The collaboration of life sciences and microfluidics companies can help in developing technologies that can also reduce development costs, speed up time-to-market and increase success rates for cost-effective commercialization. During this talk, examples of industrial solutions and recent technology developments will be presented, with special focus on the life sciences sector.

15:00

Lorena DiéguezSara Abalde-CelaKeynote Presentation

Optofluidic Devices For Disease Diagnosis
Lorena Diéguez, Leader of the Medical Devices Research Group, INL- International Iberian Nanotechnology Laboratory
Sara Abalde-Cela, Staff Researcher, International Iberian Nanotechnology Laboratory, Portugal

The capacity of microfluidic platforms as tiny labs transitioned from a potential to a reality as demonstrated in the past 20 years. The microfluidics community developed extremely advanced systems able to perform almost any chemical and/or biological lab protocol. However, in order to develop integrated lab-on-a-chip diagnostic platforms, it is crucial to couple microfluidic devices with detection strategies able to match the inherent properties of microfluidics: high-throughput, automation and miniaturization. One of those detection techniques is surface-enhanced Raman scattering (SERS) spectroscopy, an ultrasensitive and highly selective analytical technique with multiplexing ability. SERS is based on the use of plasmonic nanoparticles that act as nanoantennas and augment the very specific Raman signatures of molecules close to the nanostructured surfaces. We will show how the integration of different SERS sensing substrates and strategies within microfluidics and microdroplets offers a great flexibility for the diagnosis of several biological species and/or events. We will show examples in the context of liquid biopsy demonstrating the single-cell multiplex phenotypic analysis in a microdroplet, the detection of panels of mutations in cancer or the differentiation of cancer versus healthy cells. These approaches may be transferred to different analytical fields, such as the detection and discrimination of foodborne pathogens, bacteria or viruses in food or water samples.

15:30

Redbud LabsMicrofluidics for Low-Throughput Assay Automation
Richard Chasen Spero, CEO, Redbud Labs

Lab automation reduces errors, improves consistency, and reduces costs. But while lab automation equipment is typically optimized for processing hundreds or thousands of samples per day, most labs work with much lower throughput—say, 10–100 samples per day, and therefore their workflows are almost always performed by hand. Redbud Labs has developed an automation platform optimized for low- to medium-throughput. We use a novel microfluidic agitation method to maximize reaction yield for sample prep and other assays, resulting in lab-quality yield, fast runtimes, and a compact design. We will report on performance of this system in a range of assays, from nucleic acid extraction to protein enrichment for immunoassays. We will also share strategies employed to develop our platform on a highly compressed timeline.

16:00

Biosensing Devices Integrating Functional Nucleic Acids for Detection of Bacterial and Viral Pathogens
John Brennan, Professor and Director, Biointerfaces Institute, McMaster University, Canada

DNA aptamers and DNA enzymes (denoted as functional nucleic acids or FNA) are an emerging platform for development of point-of-care (POC) diagnostic devices.  In this presentation, I will first focus on the development of new DNA aptamers and DNA enzymes for a range of key biomarkers and their integration into colorimetric, fluorimetric and electrochemical assays for detection of infectious diseases.  Methods to transduce the binding of a target to a FNA into the production of a DNA strand as an output will then be described.  The use the output DNA to directly initiate color production, generate test lines on lateral flow devices, or produce room temperature isothermal amplification through rolling circle amplification methods will then be outlined.  Finally, the integration of the FNA assays into paper devices and electrochemical sensors will be described as platform for a range of new POC devices that allow facile detection of clinical analytes.  Examples will be provided outlining paper-based devices for ultra-sensitive detection of bacteria such as Escherichia coli, Clostridium difficile, Helicobacter pylori,  Staphylococcus Aureus and SARS-CoV-2 will be described.

16:30

JOANNEUM RESEARCH Forschungsgesellschaft mbHNovel Origami Foil Based Microfluidic Foils for DNA Amplification and Multiplex Detection Fabricated by R2R UV NIL
Anne Linhardt, Scientist, JOANNEUM RESEARCH Forschungsgesellschaft mbH

We present the implementation of DNA amplification by LAMP as well as multiplex DNA detection using the same polymer foil based microfluidic chip. This is accomplished through our unique pilot line for high throughput production of microfluidic systems based on Roll-to-Roll (R2R) processes.

16:45

JOANNEUM RESEARCH Forschungsgesellschaft mbHUpscaling of Microfluidic Biosensors Manufacturing by Imprinting, Printing and Lamination Processes on Polymer Foils
Anja Haase, Senior Scientist, JOANNEUM RESEARCH Forschungsgesellschaft mbH

High throughput fabrication based on roll-to-roll UV imprint lithography of lab-on foil chips for protein or DNA detection is presented. Subsequent fabrication process steps, such as biomolecule spotting and chip lamination are also roll-to-roll based.

17:00

Microfluidic Chip For In Vitro Neuronal Cell Culture Under Electrical Stimulation
Ana Ayerdi , Senior Researcher, Tecnalia Research & Innovation, Spain

A chip with a fluidic design that allows neuronal cell culture and axon growth monitoring was developed. This design consists of two separated chambers in which cells are seeded and allows the monitoring of axons outgrowth through the microchannels. Using industrial compatible processes, we have fabricated polymer shims and working stamps to manufacture such patterned surfaces both in a small scale plate to plate fashion as well as continuous high throughput roll-to-roll fabrication. The material choice allows for fluorescence microscopy imaging. The design was screened in vitro with different neuronal like cell lines and using several substrates that can be used for high throughput roll-to-roll manufacturing. The design is also compatible with a custom-made electrical stimulus generator. Neuronal cells respond to electrical stimulation in two ways: by increasing their cell size and by increasing the cell number in culture. With this approach chips that can be manufactured in a faster and roll to roll fabrication method for in vitro drug screening assays will be shown.

18:00

Networking Reception with Beer and Wine: Network with Colleagues and Engage with Exhibitors and Conference Sponsors

19:00

Close of Programming on Day 1 of the Conference

Wednesday, 22 June 2022


Please Refer to the POC Diagnostics Agenda for Details of Programming on Wednesday, 22 June 2022


Please Also Refer to the Organ-on-a-Chip Track Parallel Agenda for Details of Programming on Wednesday, 22 June 2022


Add to Calendar ▼2022-06-21 00:00:002022-06-22 00:00:00Europe/LondonLab-on-a-Chip and Microfluidics Europe 2022Lab-on-a-Chip and Microfluidics Europe 2022 in Rotterdam, The NetherlandsRotterdam, The NetherlandsSELECTBIOenquiries@selectbiosciences.com