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SELECTBIO Conferences Bioprinting and Bioink Innovations for 3D-Tissues

Bioprinting and Bioink Innovations for 3D-Tissues Agenda

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Wednesday, 6 October 2021


Shaochen ChenConference Chair

Conference Welcome and Introduction by Conference Chairperson: Topics Covered and Themes Addressed in this Web Conference
Shaochen Chen, Professor, The University of California San Diego, United States of America


3D Bioprinting of Living Tissues and Organs
Ibrahim Ozbolat, Hartz Family Associate Professor of Engineering Science and Mechanics, The Huck Institutes of the Life Sciences, Penn State University, United States of America

3D Bioprinting is a disruptive technology enabling deposition and patterning of living cells in order to manufacture replacement tissues and organs for tissue engineering, regenerative medicine, disease modeling and drug screening purposes. In this talk, Dr. Ozbolat will survey the emerging field of bioprinting and its impact on medical sciences. In the first part of his seminar, he will present a wide range of 3D bioprinting efforts in manufacturing of tissue/organ substitutes performed in his laboratory in the last nine years. In the second part, he will present a new bioprinting technique, called aspiration-assisted bioprinting, and explain the underlying physical mechanism in order to understand the interactions between physical governing forces and aspirated viscoelastic tissue building blocks. Finally, he will demonstrate a new intraoperative bioprinting approach in order to repair composite soft/hard tissues during craniofacial reconstruction on a rat model in a surgical setting.


Michael McAlpineKeynote Presentation

3D Printing Bionic Devices
Michael McAlpine, Kuhrmeyer Family Chair Professor of Mechanical Engineering, University of Minnesota, United States of America

The ability to directly print biomedical devices on the body could benefit patient monitoring, wound treatment, and even allow for the possibility of human augmentation. This concept requires the 3D printer to adapt to the various translations, rotations, and deformations of the biological surface. Conventional 3D printing technologies typically rely on open-loop, calibrate-then-print operation procedures. An alternative approach is adaptive 3D printing, which is a closed-loop method that combines real-time feedback control and direct ink writing of functional materials in order to fabricate devices on moving freeform surfaces. Here we demonstrate that the changes of states in the 3D printing workspace in terms of the geometries and motions of target surfaces can be perceived by an integrated robotic system aided by computer vision. This allows us to directly 3D print a wireless antenna based on a novel silver ink a free-moving human hand, to power a skin-mounted LED. Moreover, we developed an in situ 3D printing system that estimates the motion and deformation of the target surface to adapt the toolpath in real time. With this printing system, a hydrogel-based sensor was printed on a porcine lung under respiration-induced deformation. The sensor was compliant to the tissue surface and provided continuous spatial mapping of deformation via electrical impedance tomography. This adaptive 3D printing approach may enhance robot-assisted medical treatments, enabling advanced medical treatments, as well as autonomous and direct printing of wearable electronics on and inside the body.


Morning Coffee Break


3D Bioprinting Physiologically Relevant Human Infarct Models and Cardiac Patches
Pinar Zorlutuna, Sheehan Family Collegiate Professor of Engineering, University of Notre Dame, United States of America

In the modern world, myocardial infarction (MI) is one of the most common cardiovascular diseases (CVDs) which are responsible for nearly 32% of all deaths causing almost 18 million people to die every year. Even though MI remains one of the leading CVDs, limited progress has been achieved with human MI models and therapeutic treatment options. 3D bioprinting has become one of the most powerful tools used to fabricate mimetic cardiac tissues, capturing the complexity of the native cellular composition and matrix structure. However, the generation of 3D tissue constructs with multiple cell types, matching mechanical properties, the ordered structure of the native extracellular matrix and the electroconductivity of the human heart remains a challenge in cardiac tissue engineering. To address the first two challenges, we developed novel bioinks combining gelatin methacryloyl (GelMA) or GelMA-methacrylated hyaluronic acid (MeHA) hydrogels with decellularized human cardiac extracellular matrix (dhECM), and characterized them in terms of mechanical, rheological, swelling, printability, and biocompatibility properties. Composite GelMA–MeHA–dhECM (GME) hydrogels demonstrated improved mechanical properties by an order of magnitude compared to the GelMA–dhECM (GE) hydrogels, which corresponds to the difference between the stiffness of healthy cardiac tissue (8–12 kPa) and scar tissue (>150 kPa) formed after myocardial infarction (MI). Knowing that, we printed an infarct region model using a dual printhead by mixing iCMs with GE to model the healthy tissue and hCFs with GME to represent the scar tissue. To address the latter challenges, we developed a new composite construct that can provide both conductive and topographical cues for iCMs by 3D printing conductive titanium carbide (Ti3C2Tx) MXene in pre-designed patterns on polyethylene glycol (PEG) hydrogels, using aerosol jet printing, at a cell-level resolution and then seeded with iCMs and cultured for one week with no signs of cytotoxicity.


Lijie Grace ZhangKeynote Presentation

4D Bioprinting Smart and Nanomaterials for Biomedical Applications
Lijie Grace Zhang, Professor and Associate Dean for Research, The George Washington University, United States of America

4D printing is an emerging additive manufacturing process to fabricate smart structures with the ability to transform over time. Our pioneering work in designing novel 4D bioprinting smart and nanomaterials has shown great promise for various biomedical applications. We have successfully designed a series of novel 4D bioprinted tissue structures with multi-responsive abilities, including internal stress-induced, solvent-responsive, thermo-responsive, and light-responsive tissue constructs. These smart constructs exhibit excellent biocompatibility and have significantly enhanced various stem cell functions when compared to traditional bioprinting materials. For instance, recently novel light-triggered 4D bioprinted tissue constructs were created via incorporating nanomaterials into our stimuli-responsive smart materials. A proof-of-concept 4D bioprinted brain model was fabricated and the resultant construct exhibited a dynamically and remotely controllable transformation triggered by light in a spatiotemporal manner, which promoted excellent neural stem cell growth and differentiation. Our studies have shown the great potential of 4D bioprinting for the development of multi-responsive smart tissues and advanced biomedical devices.


Allegro 3D, Inc.Direct In-well Bioprinting Process and Bioinks
Wei Zhu, CEO and Co-Founder, Allegro 3D, Inc.


Lunch Break


Biofabrication of Neural Microphysiological Systems
Michael Moore, Professor of Biomedical Engineering, Tulane University and Co-Founder, AxoSim, United States of America

Microphysiological systems are being aggressively pursued as models of diseases for research and for screening drugs to better predict safety and efficacy on the path toward clinical trials. While a large number and wide variety of such systems abound, there has been relatively little progress toward the development of microphysiological models of peripheral nerve. This may be due in part because it is challenging to model the physiology of peripheral nerve in a manner that results can be interpreted in the context of human nerve function and/or disease. We have developed and reduced to commercial practice a 3D model of peripheral nerve in which axonal conduction is used as a primary functional metric, which is analogous to nerve conduction testing performed clinically. We have expanded on this recently to introduce a model of synaptic transmission from peripheral nerve to the dorsal spinal cord. This model may prove useful in the search for the next generation of pain-relieving drugs by being able measure effectiveness and parse mechanisms of different compounds without relying solely on behavioral studies in animals. As neural microphysiological models are increasingly pursued as viable commercial drug development strategies, scale-up of both fabrication and testing is rapidly becoming a major concern. We have begun to explore some unique biofabrication methods to begin to address this challenge.


Ali KhademhosseiniKeynote Presentation

Engineering in Precision Medicine
Ali Khademhosseini, CEO and Founding Director, Terasaki Institute for Biomedical Innovation, United States of America

Engineered materials that integrate advances in polymer chemistry, nanotechnology, and biological sciences have the potential to create powerful medical therapies. Dr. Khademhosseini is interested in developing ‘personalized’ solutions that utilize micro- and nanoscale technolgoies to enable a range of therapies for organ failure, cardiovascular disease and cancer.In enabling this vision he works closely with clinicians (including interventional radiologists, cardiologists and surgeons). For example, he has developed numerous techniques in controlling the behavior of patient-derived cells to engineer artificial tissues and cell-based therapies. His group also aims to engineer tissue regenerative therapeutics using water-containing polymer networks called hydrogels that can regulate cell behavior. Specifically, he has developed photo-crosslinkable hybrid hydrogels that combine natural biomolecules with nanoparticles to regulate the chemical, biological, mechanical and electrical properties of gels. These functional scaffolds induce the differentiation of stem cells to desired cell types and direct the formation of vascularized heart or bone tissues. Since tissue function is highly dependent on architecture, he has also used microfabrication methods, such as microfluidics, photolithography, bioprinting, and molding, to regulate the architecture of these materials. He has employed these strategies to generate miniaturized tissues. To create tissue complexity, he has also developed directed assembly techniques to compile small tissue modules into larger constructs. It is anticipated that such approaches will lead to the development of next-generation regenerative therapeutics and biomedical devices.


Panel Discussion Chaired by Professor Chen - Round-Table Discussion and Q&A Section on Emerging Technologies in Bioprinting and Biofabrication


Afternoon Break

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