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SELECTBIO Conferences Lab-on-a-Chip and Microfluidics Europe 2023


In situ Quantification of Osmotic Pressure within Developing Tissues

Antoine Vian, PostDoctoral Scholar, Physics of Life

Mechanics is known to play a fundamental role in many cellular and developmental processes. Beyond active forces and material properties, osmotic pressure is believed to control essential cell and tissue characteristics. However, it remains very challenging to perform in situ and in vivo measurements of osmotic pressure. Here we introduce double emulsion droplet sensors that enable local measurements of osmotic pressure intra- and extra-cellularly within 3D multicellular systems, including living tissues. After generating and calibrating the sensors, we measured the osmotic pressure in blastomeres of early zebrafish embryos as well as in the interstitial fluid between the cells of the blastula by monitoring the size of droplets previously inserted in the embryo. Our results show a balance between intracellular and interstitial osmotic pressures, with values of approximately 0.7 MPa, but a large pressure imbalance between the inside and outside of the embryo. The ability to measure osmotic pressure in 3D multicellular systems (developing embryos, organoids, etc.) will help understand its role in fundamental biological processes.

Add to Calendar ▼2023-06-19 00:00:002023-06-20 00:00:00Europe/LondonLab-on-a-Chip and Microfluidics Europe 2023Lab-on-a-Chip and Microfluidics Europe 2023 in Rotterdam, The NetherlandsRotterdam, The