Shopping Cart (0)
My Account

Shopping Cart
SELECTBIO Conferences Translational Regenerative Medicine Congress 2014


Potential of Autologous 3D Adipose Stem Cells Graft for Bone Reconstruction

Denis Dufrane, Professor, Catholic University of Louvain

A three-dimensional (3D) osteogenic graft from autologous adipose mesenchymal stem cells (AMSCs) was assessed for clinical feasibility, safety, and efficacy for long bone non-union reconstruction. AMSC isolation and differentiation into a 3D “bone-like” structure was performed for (i) three patients with bone tumour and (ii) three patients with bone pseudarthrosis. The subcutaneous adipose tissue was procured by a simple aspiration. A mean of 3•7 ± 0•9 months was required to achieve the 3D osteogenic graft as characterized by the presence of osteoblasts and a mineralization phenotype. No native tumour anomalies were found prior to or after osteogenic differentiation of AMSCs. Although AMSC expansion can induce aneuploidies, such as tri- or tetraploidies or clonal trisomy 7 in 6%–20% of cells, the osteogenic differentiation significantly reduced these clonal anomalies. The graft was implanted in the bone defect without any fixation material or scaffold.No acute (<3 months) side effects, such as impaired wound healing, pain, inflammatory reaction, and infection, or long-term side effects (>1 year), such as tumour development, were associated with the graft up to a maximum of 3 years post-transplantation. Rapid bone consolidation was demonstrated after 3 months without reversibility despite the pathophysiological context of congenital pseudarthrosis and chemotherapy. This first human trial demonstrated (i) the reproducibility of obtaining a 3D structure from autologous AMSCs and (ii) the safety and efficacy of this clinical procedure in stringent and extreme conditions. This technology should be tested on a larger scale for indications requiring bone reconstruction.

Add to Calendar ▼2014-10-21 00:00:002014-10-22 00:00:00Europe/LondonTranslational Regenerative Medicine Congress 2014Translational Regenerative Medicine Congress 2014 in Leipzig, Germany Leipzig, Germany