Monday, 21 March 2022

00:00

Jordan MillerKeynote Presentation

Title to be Confirmed
Jordan Miller, Assistant Professor of Engineering and Founder of the Advanced Manufacturing Research Program, Rice University, United States of America

00:00

Engineering Organ-Specific Microvasculature for Regeneration and Disease Modeling
Ying Zheng, Associate Professor, Department of Bioengineering, University of Washington, United States of America

Engineered tissues have emerged as promising new approaches to repair damaged tissues as well as to provide useful platforms for drug testing and disease modeling. Obstacles remain to recreate organ-specific vasculature and tissue environment for disease modeling. In this talk, I will present our progress in generating complex vascular structure with large range of diameters and curvatures, by combining multiple fabrication tools, to support its remodeling, thick tissue growth and blood perfusion. I will present means to study organ-specific vascular structure and function. I will summarize the remaining challenges and perspective in exploiting engineering tools to advance our understanding of biology and medicine.

00:00

Thomas BollenbachKeynote Presentation

Title to be Confirmed
Thomas Bollenbach, Chief Technology Officer, Advanced Regenerative Manufacturing Institute (ARMI, BioFabUSA, United States of America

00:00

Wai Yee YeongKeynote Presentation

Title to be Confirmed.
Wai Yee Yeong, Programme Director and Associate Professor, Nanyang Technological University, Singapore

00:00

Yan Yan Shery HuangKeynote Presentation

Cellular Dynamics and ‘MOrPF-genesis’ in Biofabrication and Bioprinting
Yan Yan Shery Huang, Assistant Professor, Department of Engineering, University of Cambridge, United Kingdom

Dynamic and time-dependent processes are inherent in living matters. With the aim to fabricate advanced biological systems and products, it is important to consider how cells dynamically interact with their ‘biofabrication’ protocol and environment. This talk will focus on three example cases. First, it will present process visualization of cell extrusion deposition through a high-resolution nozzle. In this context, we observed the motions of cells were overwhelmed by cellular re-organization events in a nozzle, including aggregation and sedimentation. Thus, the cells do not follow the flow paths purely driven by a laminar fluid flow within a narrow tip. In the second example, the talk will present how cells could dynamically adjust their shapes during migration, in response to different microfibre patterns from straight to curly. Key morphological features such as the variation of cells’ minor axis were identified for understanding cell migration in fibril matrices. In the third example, it will present how integrating self-assembly and biofabrication could lead an organoid engineering approach termed Multi-Organoid Patterning and Fusion (MOrPF). MOrPF is used to assemble scaffold-free macroscale airway tubes, leading to flowable organoid-on-a-chip, and branching tubular structures. Together, our studies might provide guiding principles for optimizing biofabrication processes of cell-laden constructs.

00:00

Biofabrication of Heart Tissues using Cardiac Spheroids and 3D Bioprinting Technology for in vitro and in vivo Applications
Carmine Gentile, Lecturer in the School of Biomedical Engineering, University of Technology Sydney, Australia

3D bioprinting technology has emerged in the past 15 years as a tool for the bioengineering of human tissues and organs. In this approach, bioinks containing tissue-specific cells are deposited within permissive hydrogels to 3D bioprint viable and functional tissues. Dr Gentile’s team has developed cardiac bioinks by co-culturing cardiac cells in 3D as cardiac spheroids, which are then embedded in specialized hydrogels. 3D bioprinted heart tissues are viable, highly vascularized and contract synchronously when electrically paced. They are currently used for in vitro drug discovery and toxicity studies, for disease modeling of myocardial damage (i.e., heart attack in a Petri dish) and for cardiac regeneration purposes in vivo. Given their unique features in recapitulating the microenvironment typical of the human heart and its pathophysiology, 3D bioprinted heart tissues have the potential to be used to both prevent and treat cardiovascular disease in patients.

00:00

Stephanie SeidlitsKeynote Presentation

Engineering Ex Vivo Models of Brain Cancer
Stephanie Seidlits, Associate Professor, Biomedical Engineering, University of Texas at Austin, United States of America

The Seidlits lab works to design matrix-mimetic biomaterials for engineering tissues of the central nervous system (CNS). We work with hyaluronic acid (HA), a major component of the extracellular matrix (ECM) in the CNS, as a base material to create ex vivo models of brain and tumor tissues. I will discuss our work modeling glioblastoma (GBM), the most lethal, yet common, cancer originating in the brain. We have developed HA-based culture platforms that provide a controlled experimental context in which to characterize how the ECM microenvironment facilitates GBM tumor aggression. These biomaterial-based cultures of patient-derived GBM cells can model several aspects of clinical tumors, including kinetics of acquired resistance to chemotherapies, metabolic changes, and vasculature-associated infiltration.