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SELECTBIO Conferences BioEngineering 2017: BioMEMS, 3D-BioPrinting & Synthetic Biology

Hsueh-Chia Chang's Biography



Hsueh-Chia Chang, Bayer Professor of Engineering, University of Notre Dame

H-C (Chia) Chang is a leader in electrokinetics, an important micro/nanofluidic technology for biochip platforms in future disease diagnostic products. His approach combines insightful theoretical analysis with simple but creative experiments to uncover new electrokinetic phenomena or to verify speculated ones. These new phenomena then led to inventions in new molecular diagnostics that use non-equilibrium electrokinetics and field-focusing physics to dramatically improve the performance of equilibrium probe-based assays without external fields. He and his PhD and postdoc students are inventors of 10 Notre Dame patents and 6 provisional patents, the largest IP output from any lab at Notre Dame. Five technologies have been licensed by startups near Notre Dame. One startup FCubed LLC will be going public this year. Another startup AgenDx has just licensed another of his technologies. Since 2000, 18 PhD and post-doc students of the Chang laboratory have embarked on academic careers as tenure-track professors at Chemical Engineering, Mechanical Engineering, Electrical Engineering, Food Science, Chemistry departments in 5 continents. Chia is the coauthor of a seminal book on Electrokinetics and he is the Founding Editor of Biomicrofluidics, the first American Institute of Physics journal in biology. He has published over 270 articles with 13,000 citations and an H-index of 63.

Hsueh-Chia Chang Image

A Solid-State Nanopore microRNA Quantification Platform

Thursday, 16 March 2017 at 11:30

Add to Calendar ▼SELECTBIOenquiries@selectbiosciences.com

We report a new highly selective (PCR-free) solid-state nanopore miRNA quantification platform for liquid biopsy and single-cell assays. A single ion-track nanopore in a PET membrane is asymmetrically etched into a conic geometry and is coated with a high-permittivity dielectric layer by Atomic Layer Deposition.  The surface modified conic nanopore allows for high throughput molecular translocation (100 Hz vs 1 Hz for protein nanopores) and selective delay of single-stranded (ss) nucleic acids compared to their hybridized double-stranded (ds) duplex (mean translocation time of 100 ms vs 1 ms with 5% overlap in the two distributions). The delay is due to enhanced van der Waal attraction between exposed rings of the ss-nucleic acids, with delocalized electrons, with the high-permittivity coating. Individual translocation events can be recorded for a mixture of ss- and ds-nucleic acids numbering between 100 to 100,000.  Whether the translocating molecule of each event is an ss miRNA or its ds duplex can be discerned with 95% confidence.

A Solid-State Nanopore microRNA Quantification Platform

Thursday, 16 March 2017 at 11:30

Add to Calendar ▼SELECTBIOenquiries@selectbiosciences.com

We report a new highly selective (PCR-free) solid-state nanopore miRNA quantification platform for liquid biopsy and single-cell assays. A single ion-track nanopore in a PET membrane is asymmetrically etched into a conic geometry and is coated with a high-permittivity dielectric layer by Atomic Layer Deposition. The surface modified conic nanopore allows for high throughput molecular translocation (100 Hz vs 1 Hz for protein nanopores) and selective delay of single-stranded (ss) nucleic acids compared to their hybridized double-stranded (ds) duplex (mean translocation time of 100 ms vs 1 ms with 5% overlap in the two distributions). The delay is due to enhanced van der Waal attraction between exposed rings of the ss-nucleic acids, with delocalized electrons, with the high-permittivity coating. Individual translocation events can be recorded for a mixture of ss- and ds-nucleic acids numbering between 100 to 100,000.  Whether the translocating molecule of each event is an ss miRNA or its ds duplex can be discerned with 95% confidence.


Add to Calendar ▼2017-03-16 00:00:002017-03-17 00:00:00Europe/LondonBioEngineering 2017: BioMEMS, 3D-BioPrinting and Synthetic BiologyBioEngineering 2017: BioMEMS, 3D-BioPrinting and Synthetic Biology in Boston, USABoston, USASELECTBIOenquiries@selectbiosciences.com