Gergana Nestorova,
James C. Jeffrey, M.D. Endowed Professorship in Pre-Med,
Louisiana Tech University
Gergana G. Nestorova is an Assistant Professor of Biological Sciences and the Program Chair for the Graduate Program in Molecular Sciences and Nanotechnology at Louisiana Tech University. Her research group develops lab-on-a-chip technologies for genomics and proteomics analysis including RNA and extracellular vesicles. Recent efforts have been focused on the validation of microprobe-based technology for genetic analysis on ISS and studies of the effects of radiation on gene alteration in exosomes.
One-Step Gene Sampler Tool for Genetic Analysis on ISS
Thursday, 30 September 2021 at 14:00
Add to Calendar ▼2021-09-30 14:00:002021-09-30 15:00:00Europe/LondonOne-Step Gene Sampler Tool for Genetic Analysis on ISSFlow Chemistry Summit 2021 in Boston, USABoston, USASELECTBIOenquiries@selectbiosciences.com
The aim to support an extended human presence in space has led to the
establishment of NASA’s GeneLab, which combines a database repository
dedicated to ISS biological experiments and corresponding ground-based
studies. The biggest constraints for real-time genetic analysis of
biological specimens in space are the time that is required for the
astronaut to process the sample and the reduced working area on ISS.
Because of these limitations, the number of samples that are currently
being analyzed in space is very low. The One-Step Gene Sampler tool can
significantly reduce the time required for genetic analysis on ISS and
therefore could increase the number of samples analyzed in space. This
presentation will discuss the design, application, and validation of
this technology on ISS. At the core of this tool is a microscopic pin
for the purification of nucleic acid that is analyzed by the WetLab-2
facility currently on station. The Gene Sampler tool can be used for RNA
purification at various locations of the biological sample and does not
require sacrificing of the specimen. Most valuably, the probes need no
further processing to separate RNA from the sample. The specimen’s RNA
hybridizes to the surface of the probe and no nuclear contamination
occurs. Sampling is completed after a minimum of two-minute insertion
into the specimen and the probe can be analyzed directly in the ISS
SmartCycler instrument. Instead of using the conventional liquid-based
process, the purification of genetic material now can be performed dry,
utilizing a functionalized metal pin that is compatible with the ISS
environment and analytical tools. The technology was launched on SpaceX
CRS-21 and validated by Dr. Kate Rubins in February 2021.
Add to Calendar ▼2021-09-30 00:00:002021-10-01 00:00:00Europe/LondonFlow Chemistry Summit 2021Flow Chemistry Summit 2021 in Boston, USABoston, USASELECTBIOenquiries@selectbiosciences.com
Add to Calendar ▼2021-09-30 14:00:002021-09-30 15:00:00Europe/LondonOne-Step Gene Sampler Tool for Genetic Analysis on ISSFlow Chemistry Summit 2021 in Boston, USABoston, USASELECTBIOenquiries@selectbiosciences.com
