Yazhen Zhu,
Assistant Project Scientist,
David Geffen School of Medicine at UCLA
Dr. Yazhen Zhu is a Pathologist who obtained her MD and PhD training in the fields of Clinical Medicine and Pathology. She is currently an Assistant Researcher in the Department of Molecular & Medical Pharmacology at UCLA David Geffen School of Medicine, working on the development of non-invasive cancer diagnostic assays. Her interest remains in bridging the gap between translational medicine and unmet clinical needs in the field of molecular pathology and oncology.
Click Chip Enables Non-Invasive mRNA Analysis in Circulating Tumor Cells
Wednesday, 9 October 2019 at 16:00
Add to Calendar ▼2019-10-09 16:00:002019-10-09 17:00:00Europe/LondonClick Chip Enables Non-Invasive mRNA Analysis in Circulating Tumor CellsSingle Cell Analysis Summit 2019 in Coronado Island, CaliforniaCoronado Island, CaliforniaSELECTBIOenquiries@selectbiosciences.com
Recent research focus in the field of circulating tumor cells (CTCs) has moved beyond the simple capture or enumeration of CTCs and gravitated towards approaches that allow for in-depth molecular analysis. CTCs house intact mRNA, providing more reliable gene signatures in tumors non-invasively. Exploring the use of CTC-derived mRNA offers a non-invasive diagnostic solution for understanding underlying tumor biology, guiding treatment intervention, and monitoring disease progression. Recently, we demonstrated a new covalent chemistry-enabled CTC capture/release platform – “Click Chip”. This platform is designed by integrating bioorthogonal ligation-mediated CTC capture (sensitive and rapid) and disulfide cleavage-driven CTC release (mild, specific, and rapid) on nanostructured substrates to enable efficient purification of CTCs with high-quality CTC-derived mRNA. The CTC-derived mRNA was subjected to RT-droplet digital PCR (RT-ddPCR) or Nanostring nCounter platform. In our proof of concept study, ALK/ROS1 rearrangements were quantified using CTC-mRNA recovered by Click Chips and matched with those identified in biopsy specimen in late-stage ALK/ROS1 positive NSCLC patients. Further, CTC-mRNA recovered from HCC patients by Click Chips can be used for HCC-specific mRNA profiling for predicting HCC prognosis. This streamlined workflow is optimum for non-invasive gene expression profiling based on high-quality CTC-derived mRNA.
Add to Calendar ▼2019-10-09 00:00:002019-10-09 00:00:00Europe/LondonSingle Cell Analysis Summit 2019Single Cell Analysis Summit 2019 in Coronado Island, CaliforniaCoronado Island, CaliforniaSELECTBIOenquiries@selectbiosciences.com
Add to Calendar ▼2019-10-09 16:00:002019-10-09 17:00:00Europe/LondonClick Chip Enables Non-Invasive mRNA Analysis in Circulating Tumor CellsSingle Cell Analysis Summit 2019 in Coronado Island, CaliforniaCoronado Island, CaliforniaSELECTBIOenquiries@selectbiosciences.com
