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SELECTBIO Conferences 3D-Bioprinting "Track B"

Joseph Kinsella's Biography

Joseph Kinsella, Assistant Professor of Bioengineering, McGill University

Joseph “Matt” Kinsella is an Assistant Professor of Bioengineering at McGill University in Montreal, QC, Canada. The Kinsella labs research interests include developing nano/microscale materials for cancer bioprinting and diagnostics. Before joining the faculty at McGill in 2012 he was an American Cancer Society Postdoctoral Fellow at the University of California, San Diego developing nanomaterials to aid in cancer diagostics and imaging. In 2007 he received his PhD from the Weldon School of Biomedical Engineering at Purdue University.

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Developing Bioink Formulations that Promote Multicellular Tumor Organoid Growth

Thursday, 4 October 2018 at 15:00

Add to Calendar ▼2018-10-04 15:00:002018-10-04 16:00:00Europe/LondonDeveloping Bioink Formulations that Promote Multicellular Tumor Organoid

The cellular, biochemical, and biophysical heterogeneity of native tissue microenvironments are not recapitulated by growing immortalized cell lines using conventional 2D cell culture. These challenges can be overcome using bioprinting techniques to build heterogeneous 3D tissue models whereby, different types of cells are embedded. Alginate and gelatin are two of the most common biomaterials employed in bioprinting due to their biocompatibility, biomimicry, and mechanical properties. By combining the two polymers we demonstrate a bioprintable composite hydrogel with likenesses to the microscopic architecture of native tissue stroma. The printability of the composite hydrogels are evaluated mechanically to acquire the optimal extrusion bioprinting timescale post-gelation. Breast cancer cells and fibroblasts embedded into the hydrogels can be printed to form a 3D model mimicking the in vivo tumor microenvironment. The bioprinted heterogeneous model achieves high viability for long-duration cell culture (>30 days) and promotes the self-assembly of the breast cancer cells into multicellular tumor spheroids (MCTSs). We observed migration of the cancer associated fibroblast cells (CAFs) with the MCTSs in this model. Using bioprinted cell culture platforms as co-culture systems we are able to develop a unique tool to study the dependence of tumorigenesis on the stroma composition. The materials developed are a continuous effort towards a physiologically relevant “universal” bioink that can be tuned to have specific initial mechanical and biochemical properties for different tissue architectures.

Add to Calendar ▼2018-10-04 00:00:002018-10-05 00:00:00Europe/London3D-Bioprinting "Track B"