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SELECTBIO Conferences Organ-on-a-Chip and Body-on-a-Chip: In Vitro Systems Mimicking In Vivo Functions "Track A"

Shaurya Prakash's Biography

Shaurya Prakash, Associate Professor, Department of Mechanical and Aerospace Engineering, The Ohio State University

Shaurya Prakash graduated with a Ph.D. in Mechanical Engineering from the University of Illinois at Urbana-Champaign in 2007 and with a BSME from the University of Arkansas, Fayetteville in 2001. He has been on the faculty at The Ohio State University since fall 2009 where he directs the Microsystems and Nanosystems Laboratory. His research focuses on developing Microsystems and Nanosystems for applications in healthcare and medical instrumentation, water purification, and renewable and alternate energy. His group addresses fundamental scientific questions while developing new technologies for problems important to modern societal needs. The research work is multi-disciplinary often bridging several fields including mechanical engineering, surface chemistry, and materials science. Prof. Prakash has published several papers including a recently authored book titled, “Nanofluidics and Microfluidics: Systems and Applications”, (Elsevier) and is on the editorial board for the Encyclopedia of Nanotechnology (Springer). His research has received significant financial support from both industry and various government agencies.

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Engineering Control for Endothelial Permeability in Microfluidic Vessel Bifurcation System

Friday, 5 October 2018 at 16:00

Add to Calendar ▼2018-10-05 16:00:002018-10-05 17:00:00Europe/LondonEngineering Control for Endothelial Permeability in Microfluidic Vessel Bifurcation

Endothelial barrier function is known to be regulated by a number of molecular mechanisms; however, the role of biomechanical signals associated with blood flow is comparatively less explored. Biomimetic microfluidic models comprised of vessel analogues that are lined with endothelial cells (ECs) have been developed to help answer several fundamental questions in endothelial mechanobiology. However, previously described microfluidic models have been primarily restricted to single straight or two parallel vessel analogues, which do not model the bifurcating vessel networks typically present in physiology. Therefore, the effects of hemodynamic stresses that arise due to bifurcating vessel geometries on ECs are not well understood. Here, we introduce and characterize a microfluidic model that mimics both the flow conditions and the endothelial/extracellular matrix (ECM) architecture of bifurcating blood vessels to systematically monitor changes in endothelial permeability mediated by the local flow dynamics at specific locations along the bifurcating vessel structure. We show that bifurcated fluid flow (BFF) that arises only at the base of a vessel bifurcation with a stagnation pressure of ~38 dyn/ cm2 and approximately zero shear stress induces significant decrease in EC permeability compared to the static control condition in a nitric oxide (NO)-dependent manner. Similarly, intravascular laminar shear stress (LSS) (3 dyn cm-2) oriented tangential to ECs located downstream of the vessel bifurcation also causes a significant decrease in permeability compared to the static control condition via the NO pathway. In contrast, co-application of transvascular flow (TVF) (~1 µm s-1) with BFF and LSS rescues vessel permeability to the level of the static control condition, which suggests that TVF has a competing role against the stabilization effects of BFF and LSS. Recent investigations have also shown use of external electrical stimulation modulates endothelium permeability. These findings introduce BFF at the base of vessel bifurcations as an important regulator of vessel permeability and suggest a mechanism by which local flow dynamics and engineering controls can potentially assist in manipulation of vascular function in vivo.

Add to Calendar ▼2018-10-04 00:00:002018-10-05 00:00:00Europe/LondonOrgan-on-a-Chip and Body-on-a-Chip: In Vitro Systems Mimicking In Vivo Functions "Track A"