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SELECTBIO Conferences Single Cell Analysis Summit 2018

Zhibo Yang's Biography

Zhibo Yang, Assistant Professor, University of Oklahoma Health Sciences Center

Dr. Yang obtained his B.S. in Geochemistry (1997) and an M.S. degree in Physical Chemistry (2000) from the University of Science and Technology of China. In 2005, he received his Ph.D. degree at Wayne State University. He conducted postdoctoral research at Pacific Northwest National Laboratory (2005–2008) and the University of Colorado, Boulder (2008–2012). In 2012, he started his independent career at the University of Oklahoma. Dr. Yang’s current research is focused on the development and application of novel microscale mass spectrometry techniques to study single cells, tissue imaging, and multicellular tumor spheroids.

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Mass Spectrometry Analysis of Live Single Cancer Stem Cells

Tuesday, 2 October 2018 at 11:15

Add to Calendar ▼2018-10-02 11:15:002018-10-02 12:15:00Europe/LondonMass Spectrometry Analysis of Live Single Cancer Stem CellsSingle Cell Analysis Summit 2018 in Coronado Island, CaliforniaCoronado Island,

Mass spectrometry (MS) is a rapidly developing area for sensitive detection and accurate identification of broad ranges of biomolecules. We have developed a miniaturized multifunctional device, the Single-probe, that can be coupled to MS to analyze live single cells. One of the ongoing projects is to study cancer stem cells (CSCs). CSCs are vital for tumor development, relapse, and metastasis. However, current research in CSC biology is largely limited by the difficulty of obtaining sufficient CSCs. Thus, using MS method to study CSCs is a promising approach. We conducted a series of metabolomic analysis of live colorectal (HCT-116) CSCs, and compared the results with HCT-116 non-stem cancer cells (NSCCs). Our results show metabolomic features of CSCs are distinctly different from NSCCs at single cell level. Particularly, CSCs have significantly higher levels of unsaturated lipids compared to NSCCs. We demonstrated two enzymes, NF-?B and ALDH1A1, regulate the lipid desaturation in CSCs. In addition, our studies indicate that metabolites involved in TCA (tricarboxylic acid) cycle are more abundant in CSCs than those in NSCCs, indicating that their major energy production pathways are drastically different. Our studies suggest that lipid desaturation and tricarboxylic acid (TCA) cycle could be potential targets for CSC-targeted treatment.

Add to Calendar ▼2018-10-02 00:00:002018-10-03 00:00:00Europe/LondonSingle Cell Analysis Summit 2018Single Cell Analysis Summit 2018 in Coronado Island, CaliforniaCoronado Island,