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SELECTBIO Conferences Lab-on-a-Chip & Microfluidics 2019: Emerging Themes, Technologies and Applications Track "A"

Steven Graves's Biography

Steven Graves, Professor, University of New Mexico; President & CEO, BennuBio Inc.

Steven W. Graves received his Ph.D. from The Pennsylvania State University in 1998, joined the National Flow Cytometry Resource at Los Alamos National Laboratory in 1999, became a Technical Staff Member in 2001, and advanced to Team Leader of Optical Spectroscopy and Instrumentation in 2007. He moved to the University of New Mexico in 2008 in the Center for Biomedical Engineering. He is now the Director of the Center for Biomedical Engineering and a Professor of Chemical and Biological Engineering, where his research focuses on the development of biomedical instrumentation, diagnostic assays, and the study of molecular assemblies. Recently, he has applied cellular micromanipulation technologies in combination with inexpensive low-power components to create small low-cost flow cytometry approaches that will be of use both in point of care settings and as in highly parallel flow cytometers. The development of highly parallel flow cytometers led to the development of BennuBio Inc., which is a flow cytometry company dedicated to high throughput cellular analysis and sorting. Another recent interest is the development of molecular assemblies to understand the principles of information transfer in biological systems. He has an h-index of 26, 60 publications, 13 patents, and several awards, including a 2007 R&D100 award for the development of Portable Acoustic Cytometry.

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Parallel Flow Cytometry Made Simple

Tuesday, 8 October 2019 at 09:30

Add to Calendar ▼2019-10-08 09:30:002019-10-08 10:30:00Europe/LondonParallel Flow Cytometry Made

Cell killing pressure and turbulence created by high linear flow velocities, as well as the stochastic arrival of particles, inherently limit any single stream flow cytometer to approximately 50,000 events per second and flow rates of about 250 microliters to a milliliter per minute. As particle size increases, these limitations worsen due to increased turbulence in the required larger flow channels, which thereby limits the achievable linear velocities and reduces analytical rate to hundreds of particles per second. These limitations prevent flow cytometry from being used efficiently in applications that range from extremely rare cell analysis to the use of large multicellular systems in pharmaceutical discovery.  Therefore, there have been many efforts to create parallel flow cytometers, which can increase analytical and volumetric rates through the use of multiple streams. However, such systems have been plagued by complexity due to the use of multiple channels, optical paths, and laser sources that make long term operation extremely difficult. Here we present a highly parallel flow cytometer that uses a multinode acoustic standing wave, a line focused laser, and a high speed sCMOS camera to create a single optical path, single laser, and single detector system that will be able to detect up to 16 colors of emitted/scattered light from up to 4 excitation lasers.

Add to Calendar ▼2019-10-07 00:00:002019-10-09 00:00:00Europe/LondonLab-on-a-Chip and Microfluidics 2019: Emerging Themes, Technologies and Applications Track "A"