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SELECTBIO Conferences Circulating Biomarkers and Extracellular Vesicles Europe 2023

David Juncker's Biography

David Juncker, Professor and Chair, McGill University

David Juncker stayed as a visiting scientist at the National Metrology Institute of Japan in Tsukuba from 1997-98. He conducted his PhD research at the IBM Zurich Research Laboratory from 1999-2002. He then pursued his studies as a Post-doc first at IBM Zurich until 2004, and then one year at the Swiss Federal Institute of Technology in Zurich (ETH). David started as an assistant professor in the Biomedical Engineering Department of McGill University in 2005, was promoted to associate professor with tenure in 2011, and became a full professor in 2016. As of early 2018, David serves as departmental chair of the Biomedical Engineering Department at McGill University.

Dr. Juncker's current interests are in the miniaturization and integration in biology and medicine, which includes the engineering and utilization of novel micro and nanotechnologies for manipulating, stimulating and studying oligonucleotides, proteins, cells, and tissues. The emerging field of nanobiotechnology, in a broad sense, is the most exciting to him, and is also key to tackle some of the major challenges in biology and medicine, for example identifying novel biomarkers for early disease diagnosis and developing low-cost point-of-care diagnostics.

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Versatile and Multiplexed Extracellular Vesicle and Single Extracellular Vesicle Analysis

Monday, 19 June 2023 at 14:30

Add to Calendar ▼2023-06-19 14:30:002023-06-19 15:30:00Europe/LondonVersatile and Multiplexed Extracellular Vesicle and Single Extracellular Vesicle AnalysisCirculating Biomarkers and Extracellular Vesicles Europe 2023 in Rotterdam, The NetherlandsRotterdam, The

Extracellular vesicles (EVs) have emerged as a fundamental cell signaling and cargo system. EV proteomic analysis face challenges such difficulty to detect internal EV proteins, difficult to normalize concentration, co-expression, and lack of reproducibility. Single EV analysis, while highly desirable, still suffers from imprecision owing to lack of a protein markers universally expressed in EVs, low fluorescence of single molecules, and low scattering signals and the ensuing difficulty to detect small EVs. Here our efforts to address these challenges will be presented including multiplexed (i) inner and outer protein profiling in EVs; (ii) co-enrichment analysis which builds on co-expression analysis and quantifies protein co-enrichment, either negative or positive, and is illustrated with >100 protein pairs in EVs from metastatic, organotropic breast cancer cell lines. Next, (iii) a single EV analysis platform for label-free interference scattering (iSCAT) on a common (fluorescence) microscope will be introduced, and sizing of EVs from ~35 nm to ~200 nm in diameter shown, outperforming existing methods such as nanoflow cytometry; (iv) the co-expression and co-enrichment profiles of ~20 proteins on single EVs will be demonstrated by combining iSCAT, fluorescence imaging, and microfluidic exchange of DNA-barcoded antibodies. These new EV analysis methods will be useful to detect, profile and analyse EVs in health and disease, and deepen our understanding of their function.

Add to Calendar ▼2023-06-19 00:00:002023-06-20 00:00:00Europe/LondonCirculating Biomarkers and Extracellular Vesicles Europe 2023Circulating Biomarkers and Extracellular Vesicles Europe 2023 in Rotterdam, The NetherlandsRotterdam, The