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SELECTBIO Conferences Bioprinting and Bioink Innovations for 3D-Tissues 2022

Jeroen Rouwkema's Biography

Jeroen Rouwkema, Associate Professor, University of Twente

Jeroen Rouwkema, PhD, got his PhD in Regenerative Medicine from the University of Twente, the Netherlands, in 2007. He received his PhD under the supervision of Prof. Clemens van Blitterswijk and gained further experience at MIT (Prof. Bob Langer), Keele University (Prof. Alicia El Haj) and Harvard Medical School (Prof. Ali Khademhosseini). He is currently an Associate Professor at the Department of Biomechanical Engineering, University of Twente, where he leads his own group focusing on vascularization of engineered tissues. He is a tissue engineer with an extensive experience in this field, having focused on the vascularization of skeletal muscle, bone, and cardiac muscle tissue. To optimize the organization and maturation of vascular networks, he combines tissue engineering technology with mechanical and chemical stimulation, computational modeling and biofabrication. He has received several personal scholarships, including a VENI scholarship (2011-2014), a Marie Sklodowska-Curie International Outgoing Fellowship (2014-2017) and an ERC Consolidator Grant (2017-2022). In 2017 he received the TERMIS EU Robert Brown Early Career Principal Investigator Award in recognition of his achievements.

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Embedded 3D Printing of Dilute Particle Suspensions into Dense Complex Cardiac Fibers

Monday, 7 November 2022 at 14:00

Add to Calendar ▼2022-11-07 14:00:002022-11-07 15:00:00Europe/LondonEmbedded 3D Printing of Dilute Particle Suspensions into Dense Complex Cardiac FibersBioprinting and Bioink Innovations for 3D-Tissues 2022 in Boston, USABoston,

In order to fabricate functional organoids and microtissues, a high cell density is generally required. As such, the placement of cell suspensions in molds or microwells to allow for cell concentration by sedimentation, is a standard method for the production of organoids and microtissues. Even though molds offer some level of control over the shape of the resulting microtissue, this control is limited as microtissues tend to compact towards a sphere after sedimentation of the cells. 3D bioprinting offers better control over the shape of the resulting structure. However, when using extrusion 3D bioprinting, the self-assembly into organoids can be hampered due to low cell densities or the presence of bio-ink components which interfere with the self-assembly process. Even though the printing of dense cell suspensions without the addition of a biomaterial component in the ink has been reported, extruding dense cellular suspensions is challenging due to potential clogging of the printing nozzle and the exposure of the cells to high shear stresses. To circumvent these limitations, we have developed  a simple and inexpensive embedded printing method to 3D print dense functional tissues using dilute (volume fraction < 10%) aqueous particle suspensions as an ink. These particles can be cells, spheroids, hydrogel beads, or combinations thereof. Using this approach, we have shown the formation of large functional and beating cardiac tissue fibers using human pluripotent stem cells.

Add to Calendar ▼2022-11-07 00:00:002022-11-08 00:00:00Europe/LondonBioprinting and Bioink Innovations for 3D-Tissues 2022Bioprinting and Bioink Innovations for 3D-Tissues 2022 in Boston, USABoston,