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SELECTBIO Conferences Single Cell Analysis Summit 2019

John Nolan's Biography

John Nolan, Professor, The Scintillon Institute

John Nolan is a Professor at The Scintillon Institute, where his research group develops and applies new technologies for cytometry. Current projects in his lab include spectral flow cytometry, single particle analysis of natural and synthetic nanoparticles, and surface enhanced Raman scattering (SERS) in cytometry. He is on the Editorial Boards of Cytometry and Current Protocols in Cytometry, a Fellow of the American Institute of Medical and Biological Engineering (AIMBE), and Past-president of the International Society for Advancement of Cytometry (ISAC).

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Single Vesicle Analysis to Understand Intercellular Communication

Wednesday, 9 October 2019 at 13:30

Add to Calendar ▼2019-10-09 13:30:002019-10-09 14:30:00Europe/LondonSingle Vesicle Analysis to Understand Intercellular CommunicationSingle Cell Analysis Summit 2019 in Coronado Island, CaliforniaCoronado Island,

Extracellular vesicles are released from cells carrying molecular cargo that can be transferred to other cells to affect their function. Understanding the diversity of EV released from cells is important for understanding their roles in basic biology and well as to develop EV-based biomarkers and therapeutics. A particular challenge in the field is methods to sensitively detect EVs and measure their cargo, as the small size and heterogeneity of EVs limit the usefulness of conventional biochemical analysis. Single vesicle analysis offers the possibility to understand the heterogeneity of EVs released from cells, and we have developed flow cytometry (FC)-based methods to detect and size EVs as small as ~75 nm and measure cargo to ~30 molecules per vesicle. We are using these tools to measure the number, size, and cargo of EVs released from cells and to study the roles of EVs in cell-cell communication.

Add to Calendar ▼2019-10-09 00:00:002019-10-09 00:00:00Europe/LondonSingle Cell Analysis Summit 2019Single Cell Analysis Summit 2019 in Coronado Island, CaliforniaCoronado Island,