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SELECTBIO Conferences Circulating Biomarkers: Cell-Free Nucleic Acids, Proteins and Rare Circulating Cells

Laura Goldberg's Biography

Laura Goldberg, Assistant Professor of Medicine, Brown University/Rhode Island Hospital

Dr. Goldberg is currently an Assistant Professor at Brown University, Providence RI, in the Division of Hematology/Oncology at Rhode Island Hospital with interests in hematopoietic stem cell biology and extracellular vesicles. She obtained her MD and PhD in Molecular Genetics and Biochemistry from the Medical Scientist Training Program at the University of Pittsburgh, completed her residency in Internal Medicine at the University of Pittsburgh and her Hematology/Oncology Fellowship at the Warren Alpert Medical School of Brown University. Her current work is focused on the cycling nature of hematopoietic stem cells and the role of circadian rhythm in modulating stem cell function and influencing extracellular vesicle-marrow cell interactions.

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Circadian Rhythm Modulates the Ability of Pulmonary-derived Extracellular Vesicles to Alter Target Marrow Cell Phenotype

Tuesday, 21 March 2017 at 16:00

Add to Calendar ▼2017-03-21 16:00:002017-03-21 17:00:00Europe/LondonCircadian Rhythm Modulates the Ability of Pulmonary-derived Extracellular Vesicles to Alter Target Marrow Cell

We are interested in how circadian rhythm influences extracellular vesicle (EV)-mediated inter-cellular communication.  To begin exploring whether circadian oscillations alter EV function, we employed a well-established in vitro system in which lung-derived EVs, when co-cultured with murine bone marrow cells, induce the bone marrow cells to express pulmonary epithelial cell-specific mRNA and protein. Using this readily manipulated in vitro system, we were able to vary the circadian time-point of both the lung harvest for EVs and the bone marrow cell harvest for target marrow cells prior to co-culture. We found that 1) EVs, when harvested from lung at distinct circadian time-points, differentially altered the expression of pulmonary epithelial specific mRNAs in target bone marrow cells in culture, and 2) altering the circadian time-point of the target whole bone marrow cells, and co-culturing with lung-derived EVs similarly resulted in statistically significant differences in pulmonary epithelial mRNA expression due to circadian oscillations of the recipient marrow cells. These data indicate that circadian rhythm is likely an important component of EV-mediated inter-cellular communication. Our ongoing work is aimed at elucidating the mechanisms by which circadian rhythm influences EV-mediated communication with bone marrow cells. We hope such studies will provide insight into the molecular mechanisms by which EVs alter the mRNA expression profile of target WBM and help optimize EV manipulations for therapeutic interventions in the future.

Add to Calendar ▼2017-03-20 00:00:002017-03-21 00:00:00Europe/LondonCirculating Biomarkers: Cell-Free Nucleic Acids, Proteins and Rare Circulating