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SELECTBIO Conferences Lab-on-a-Chip and Microfluidics Europe 2018

Gijs Wuite's Biography

Gijs Wuite, Professor, Vrije Universiteit Amsterdam

Prof. Gijs Wuite obtained his PhD in biophysics in 2000. Since 2001 he leads his own group at the VU University Amsterdam, in 2009 was appointed to full professor and since 2014 he is head of the physics department. In his research he has successfully applied quantitative physical tools to investigate fundamental problems in biology, and to search for the unification of apparently unrelated biological phenomena. Moreover, he has been at the front of recent new and fast developments of biophysical techniques that have enabled visualization, manipulation and control of complex biological reactions. Based on this research work he founded in 2014 a company (LUMICKS) that sell the technology he and his group has developed. His work has appeared in journals such as Nature, Science, PNAS and Physical Review Letters. His research has been awarded with the prestigious personal VIDI, VICI and ERC grants. In 2009 Wuite was appointed member of the Young Academy, an independent platform of young top scientists within the Royal Netherlands Academy of Arts and Sciences.

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Frontier in Direct Observation and Visualization of Molecular Interactions

Wednesday, 6 June 2018 at 13:30

Add to Calendar ▼2018-06-06 13:30:002018-06-06 14:30:00Europe/LondonFrontier in Direct Observation and Visualization of Molecular InteractionsLab-on-a-Chip and Microfluidics Europe 2018 in Rotterdam, The NetherlandsRotterdam, The

The genetic information of an organism is encoded in the base pair sequence of its DNA. Many specialized proteins are involved in organizing, preserving and processing the vast amounts of information on the DNA. In order to do this swiftly and correctly these proteins have to move quickly and accurately along and/or around the DNA constantly rearranging it. In order to elucidate these kind of processes we perform single-molecule experiments on model systems such as restriction enzymes, DNA polymerases and repair proteins. The data we use to extract forces, energies and mechanochemistry driving these dynamic transactions. The results obtained from these model systems are then generalized and thought to be applicable to many DNA-protein interactions. In particular, I will report on a new single molecule methods. I’ll introduce Acoustic Force Spectroscopy (AFS). With AFS we extend the force-spectroscopy toolbox with an acoustic manipulation device that allows exerting acoustic forces on tethered molecules. AFS is a Lab-on-a-chip device consisting of a flow cell of two glass plates with a fluid chamber in between and a piezo element glued on top. While applying an alternating voltage to the piezo element, forces from sub-pN to hundreds of pNs are exerted to thousands of biomolecules in parallel, with sub-millisecond response time and inherent stability. AFS distinguishes itself by its relative simplicity, low cost and compactness, which allow straightforward implementation in lab-on-a-chip devices. I’ll discuss the application of this technique to DNA-protein interaction as well as for antibody-protein interaction detection and for its use to quantify cell adhesion.

Add to Calendar ▼2018-06-05 00:00:002018-06-06 00:00:00Europe/LondonLab-on-a-Chip and Microfluidics Europe 2018Lab-on-a-Chip and Microfluidics Europe 2018 in Rotterdam, The NetherlandsRotterdam, The