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SELECTBIO Conferences Point-of-Care, Biosensors & Mobile Diagnostics Europe 2019

Rosanne Guijt's Biography

Rosanne Guijt, Professor, Smart Sensing, Deakin University

Rosanne completed her undergraduate degree in Biopharmaceutical Sciences at Leiden University, the Netherlands, and her PhD degree from Delft University of Technology. Her PhD involved the development of miniaturized total analytical systems (µTAS) for real-time monitoring of fermentations, and focused on the development of devices for capillary electrophoresis with integrated conductivity detection. Her research focus expanded towards the development of portable and field deployable analytical instrumentation for a wide range of applications. She has also pioneered the use of 3D printing in microfluidics. Funded by the Dutch Science and Technology Foundation STW, she initiated Lab on a Chip research at the University of Tasmania (µTAS@UTAS). Following a postdoctoral fellowship from the Australian Research Council, she moved to the School of Medicine and was hosted as Alexander von Humboldt Fellow at the Korean Institute for Science and Technology – Europe in Saarbrucken and the Microfluidic ChipShop, Jena). Since July 2017, she has been employed as Professor, Smart Sensing, at Deakin University. She has published over 90 articles in peer-reviewed journals and het work is well cited; her h-index of 29.

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Manufacturable Microfluidic Devices – Fine Tuning the Biochemistry and Fabrication

Wednesday, 19 June 2019 at 11:00

Add to Calendar ▼2019-06-19 11:00:002019-06-19 12:00:00Europe/LondonManufacturable Microfluidic Devices – Fine Tuning the Biochemistry and FabricationPoint-of-Care, Biosensors and Mobile Diagnostics Europe 2019 in Rotterdam, The NetherlandsRotterdam, The

Manufacturability is one of the bottlenecks in the development of commercially viable microfluidic systems. As part of the development of a sample in/answer out assay for the detection of Avian influenza (H1N1) as example, different strategies were used to aid manufacturability. To simplify hardware and minimize thermal stress, isothermal amplification strategies were preferred, and Nucleic Acid Sequence Based Amplification (NASBA) was selected as amplification process because of its high fidelity in replicating RNA. Despite the isothermal amplification at 42C, this is preceded by a thermal annealing step >60C. Chemical and biochemical approaches of circumventing this thermal denaturation step will be compared. The simplified assay uses an external heater, moving magnet for RNA transport and LED induced fluorescence detection, all accessing eh amplification chamber. To minimize geometrical constraint, a dedicated mould was designed for injection moulding of a smooth and straight surface, facilitating side illumination. The described assay facilitated the detection of H1N1 down to ct28.

Add to Calendar ▼2019-06-18 00:00:002019-06-19 00:00:00Europe/LondonPoint-of-Care, Biosensors and Mobile Diagnostics Europe 2019Point-of-Care, Biosensors and Mobile Diagnostics Europe 2019 in Rotterdam, The NetherlandsRotterdam, The