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SELECTBIO Conferences Point-of-Care, Biosensors & Mobile Diagnostics Europe 2019

Rosanne Guijt's Biography

Rosanne Guijt, Professor, Deakin University

Prof Guijt specializes in microfluidic systems for chemical separation and analysis, and has pioneered the area of functionally integrated devices through the incorporation of electrodes and membranes. Currently a Professor at Deakin University, Geelong, Australia, Rosanne studied Biopharmaceutical Sciences at Leiden University, before commencing her PhD at Delft University of Technology, the Netherlands. After graduation, she received a fellowship from the Dutch Science and Technology Foundation STW to initiate Lab on a Chip research at the University of Tasmania in 2003, followed by a prestigious Australian Research Council Postdoctoral Fellowship (2004-2009) and a Alexander von Humboldt Fellowship for Experienced Researchers (2014/15 an 2016/17). Since 2014, she has led the adoption of 3D printing as an alternative fabrication approach in microfluidics.

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Manufacturable Microfluidic Devices – Fine Tuning the Biochemistry and Fabrication

Wednesday, 19 June 2019 at 11:00

Add to Calendar ▼2019-06-19 11:00:002019-06-19 12:00:00Europe/LondonManufacturable Microfluidic Devices – Fine Tuning the Biochemistry and FabricationPoint-of-Care, Biosensors and Mobile Diagnostics Europe 2019 in Rotterdam, The NetherlandsRotterdam, The

Manufacturability is one of the bottlenecks in the development of commercially viable microfluidic systems. As part of the development of a sample in/answer out assay for the detection of Avian influenza (H1N1) as example, different strategies were used to aid manufacturability. To simplify hardware and minimize thermal stress, isothermal amplification strategies were preferred, and Nucleic Acid Sequence Based Amplification (NASBA) was selected as amplification process because of its high fidelity in replicating RNA. Despite the isothermal amplification at 42C, this is preceded by a thermal annealing step >60C. Chemical and biochemical approaches of circumventing this thermal denaturation step will be compared. The simplified assay uses an external heater, moving magnet for RNA transport and LED induced fluorescence detection, all accessing eh amplification chamber. To minimize geometrical constraint, a dedicated mould was designed for injection moulding of a smooth and straight surface, facilitating side illumination. The described assay facilitated the detection of H1N1 down to ct28.

Add to Calendar ▼2019-06-18 00:00:002019-06-19 00:00:00Europe/LondonPoint-of-Care, Biosensors and Mobile Diagnostics Europe 2019Point-of-Care, Biosensors and Mobile Diagnostics Europe 2019 in Rotterdam, The NetherlandsRotterdam, The