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SELECTBIO Conferences 3D-Models for Drug Testing: Organoids & Tissue Chips 2022

Haley Pugsley's Biography

Haley Pugsley, Manager and Senior Scientist, Luminex Corporation

Haley R. Pugsley, Ph.D., is a Manager, Senior Scientist for the Amnis Flow Cytometry products at Luminex Corporation-A DiaSorin Company. Haley received her Ph.D. in Analytical Chemistry from the University of Washington working under the direction of Professor Norman J. Dovichi. At the University of Washington, she used capillary electrophoresis to study protein fingerprinting of breast cancer tissue and created two reporter cell lines to investigate the expression of RecA in Deinococcus radiodurans. Dr. Pugsley did her postdoctoral fellowship at Hematologics, Inc. under the direction of Dr. Michael R. Loken where she focused on clinical flow cytometry and its use in diagnosing hematologic malignancies. Dr. Pugsley joined Amnis (now part of Luminex Corporation-A DiaSorin Company) in 2011 developing applications for the ImageStreamX Mk II, FlowSight and CellStream flow cytometers. During her time at Luminex, Dr. Pugsley has worked on many applications including autophagy, immunological synapse, algae biomass, cell signaling, an automated method for leukemia detection using multi-parametric analysis of imaging flow cytometry data and most recently extracellular vesicles.

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Applications for Small Particle Analysis Using High Sensitivity Flow and Imaging Flow Cytometry

Tuesday, 13 September 2022 at 17:30

Add to Calendar ▼2022-09-13 17:30:002022-09-13 18:30:00Europe/LondonApplications for Small Particle Analysis Using High Sensitivity Flow and Imaging Flow Cytometry3D-Models for Drug Testing: Organoids and Tissue Chips 2022 in

Research in the fields of virology, microbiology, nanoparticles, and extracellular vesicles (EVs) has shown tremendous growth over the past few years. Viruses, which range in size from 17 nm to ~1.5 microns, small bacteria, nanoparticles, and small EVs such as exosomes (less than 150 nm in diameter), are all on the subcellular scale. Quantifying and characterizing small particles in a reproducible and reliable manner is challenging due to their small size. In this presentation, we will demonstrate the capabilities for small particle applications from Amnis® CellStream® and Amnis® ImageStream®X Mk II. The Amnis CellStream system has the advantage of high throughput flow cytometry with higher sensitivity to small particles due to the CCD-based, time-delay-integration image capturing system. Here, we will present immunophenotyping data from EVs. The Amnis ImageStreamX Mk II that combines the quantitative power of flow cytometry with microscopy in one system has a High Gain mode to increase the sensitivity for small particles by adjusting the CCD-camera to a higher gain setting, increasing the signal obtained from the small particles while minimizing the noise. In this portion of the presentation, examples of High Gain mode using murine leukemia virus-sfGFP will be shown.

Add to Calendar ▼2022-09-13 00:00:002022-09-14 00:00:00Europe/London3D-Models for Drug Testing: Organoids and Tissue Chips 20223D-Models for Drug Testing: Organoids and Tissue Chips 2022 in