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SELECTBIO Conferences Circulating Biomarkers and Extracellular Vesicles Europe 2024

Terry Morgan's Biography

Terry Morgan, Professor of Pathology and Laboratory Medicine, Oregon Health and Science University

Dr. Morgan is a Professor of Pathology and Biomedical Engineering at Oregon Health & Science University (OHSU) in Portland, Oregon, USA. He has been an NIH-funded investigator since 2012 and he has worked with BD Biosciences since 2016 to develop, validate, and begin research trials using nanoscale high resolution flow cytometry to image, count, and isolate extracellular vesicles (EVs). Dr. Morgan is the leader of the EV research group at the Center for Developmental Health at OHSU. He is also the leader of the flow cytometry EV group at the Knight Cancer Institute to test its potential as a diagnostic platform for early cancer detection.

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NanoFACS Isolated Placental Cell- and Size-Specific EVs and T-cell Polarization

Monday, 24 June 2024 at 18:00

Add to Calendar ▼2024-06-24 18:00:002024-06-24 19:00:00Europe/LondonNanoFACS Isolated Placental Cell- and Size-Specific EVs and T-cell PolarizationCirculating Biomarkers and Extracellular Vesicles Europe 2024 in Rotterdam, The NetherlandsRotterdam, The

How the placenta suppresses the maternal immune response to this invasive fetal organ is a long-standing mystery. New data suggest that placental extracellular vesicles (EVs) isolated from first trimester placental explants in vitro induce immune tolerance (ie; Bai K et al, 2022).  This is similar to Treg induction by cancer EVs, but shares the method-based weakness of testing heterologous fractions of EVs from many different cell sources.  A significant advance would be to isolate placental cell- and size-specific EVs and culture them with T-cells to determine the dose-response effect. Moreover, an important negative control would be to test isolated liposomes from the same plasmas.  In this study, we hypothesize that placental invasive extravillous trophoblast EVs induce T-cell polarization from Th1 to Tregs compared with liposomes isolated from the same plasma samples.  We isolated placental EVs and spiked-in 200nm liposomes by nanoFACS from banked human first trimester plasma samples.  T-cell polarization cultures from banked PBMCs (positive control was IL-2 induced +/- TGF-b/retinoic acid) were validated and then compared with banked PBMCs treated with either placental EVs or liposomes. Our results show that placental EVs have PD-L1 on their surfac and compared with liposomes incubated with Th1 cells show significantly more Treg and Th2 polarization after 3 days in culture.

Add to Calendar ▼2024-06-24 00:00:002024-06-25 00:00:00Europe/LondonCirculating Biomarkers and Extracellular Vesicles Europe 2024Circulating Biomarkers and Extracellular Vesicles Europe 2024 in Rotterdam, The NetherlandsRotterdam, The