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SELECTBIO Conferences Organ-on-a-Chip and Body-on-a-Chip: In Vitro Systems Mimicking In Vivo Functions "Track A"

Kara McCloskey's Biography

Kara McCloskey, Associate Professor, University of California-Merced

Kara E. McCloskey, PhD, is a Founding and Associate Professor in the School of Engineering at the University of California, Merced. She received her BS and an MS in Chemical Engineering from The Ohio State University and her PhD through a joint program with Cleveland Clinic Foundation’s Biomedical Engineering Department and Ohio State University. She then completed her postdoctoral training in vascular stem cell and tissue engineering with Dr. Robert Nerem at the Georgia Institute of Technology. Her research is in the field of cardiovascular tissue engineering with a specific focus on deriving functional cell products from stem cells. Dr. Kara McCloskey has over 15 years of experience in the area of endothelial cell (EC) fate and cardiovascular tissue engineering.

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Leaf-Inspired Microvascular Patterns

Friday, 5 October 2018 at 16:30

Add to Calendar ▼2018-10-05 16:30:002018-10-05 17:30:00Europe/LondonLeaf-Inspired Microvascular

The vascularization of tissue grafts is critical for maintaining viability of the cells within a transplanted graft.  A number of strategies are currently being investigated including very promising microfluidics systems. We explored the potential for generating a vasculature-patterned endothelial cells (EC) that could be integrated into distinct layers between sheets of primary cells.  Bioinspired from the leaf veins, we generated a reverse mold with a fractal vascular-branching pattern that models the unique spatial arrangement over multiple length scales that precisely mimic branching vasculature. By coating the reverse mold with 50µg/ml of fibronectin and stamping enabled selective adhesion of the human umbilical vein endothelial cells (HUVECS) to the patterned adhesive matrix, we show that a vascular-branching pattern can be transferred by microcontact printing.  Moreover, this pattern can be maintained transferred to a 3D hydrogel matrix and remains stable for up to 4 days. After 4 days, HUVECs can be observed migrating and sprouting into Matrigel. These printed vascular branching patterns, especially after transfer to 3D hydrogels, provide a viable alternative strategy to the prevascularization of complex tissues.

Add to Calendar ▼2018-10-04 00:00:002018-10-05 00:00:00Europe/LondonOrgan-on-a-Chip and Body-on-a-Chip: In Vitro Systems Mimicking In Vivo Functions "Track A"