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SELECTBIO Conferences High Content Analysis

Olivier Frey's Biography

Olivier Frey, Product Manager Microphysiological Systems, InSphero AG, Schlieren, Switzerland

Olivier Frey leads the Technology & Platforms group at InSphero AG, Switzerland. Before joining InSphero, Olivier Frey, was group leader at the Department of Biosystems Science and Engineering of ETH Zurich, Switzerland. In the Bio Engineering Laboratory of Prof. Andreas Hierlemann he was responsible for the development of integrated microfluidic systems for single cell handling and 3D tissue cultures. Included are in particular multi-tissue systems, or so-called “Body-on-a-Chip” configurations based on 3D microtissue spheroids for microtissue culturing, analysis and interaction. Olivier Frey received his Diploma in Mechanical Engineering from ETH Zurich and his Doctoral degree in Micro Engineering from EPF Lausanne, Switzerland, Laboratory (SAMLAB) of Prof. Nico de Rooij.

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Microfluidic Platform for 3D Spherical Microtissues: Culturing and Imaging Approaches for Multi-Cell-Based Assays

Wednesday, 14 May 2014 at 14:15

Add to Calendar ▼2014-05-14 14:15:002014-05-14 15:15:00Europe/LondonMicrofluidic Platform for 3D Spherical Microtissues: Culturing and Imaging Approaches for Multi-Cell-Based AssaysHigh Content Analysis in Barcelona, SpainBarcelona,

In-vitro cell-based assays play a key role in the overall process of drug discovery and can provide essential information on the efficacy and toxicity of a new compound. In order to increase the predictability of such assays, 3-dimensional tissue constructs receive more and more attention, as their organotypic nature is better suited to study complex physiological processes than that of 2-dimensional cell cultures, which is not only dependent on the fabrication method of 3-dimensional tissue structures, as the utilized microenvironment also strongly influences long-term viability and functionality. In our lab, we focus on spherical microtissues, produced by the hanging drop technology, as they offer two key advantages over other cell culture formats that have been used in conjunction with microfluidic networks for cell-based assays: First, they are comparably simple and reproducible to fabricate, and possess organotypic functionality and biomimetic morphology. Second, their spherical shape and compact constitution, as well as their precisely controllable size make them ideal candidates for handling in microfluidic structures in contrast to 3D-hydrogel or scaffold-based cell-cultures. Combining the advantages of spheroids and the technical capabilities of microfluidic engineering offers the possibility to develop a modular platform that accommodates multiple tissues of different cell types (e.g. tumor, liver, heart). Dedicated culturing compartments host the microtissues, and fluidic interconnections between these compartments allow for mimicking the physiological context and conditions of the human body. Further, microfabrication technologies allow the direct integration of microelectrodes for tissue monitoring via electrical impedance spectroscopy, a label-free and complementary read-out to optical microscopy.

Add to Calendar ▼2014-05-14 00:00:002014-05-15 00:00:00Europe/LondonHigh Content AnalysisHigh Content Analysis in Barcelona, SpainBarcelona,