Poster Presentations
|
Fabrication of 3D printing based hybrid scaffold including PCL, ceramic powder, and collagen for bone regeneration and its in-vivo efficacy in a rabbit
Lee JeongSeok, Student, Korea Polytechnic University
The three-dimensional (3D) printed polycaprolactone (PCL) scaffolds have been widely used to regeneration bone. However, the scaffold consisting of only PCL has a limitation to promote bone regeneration due to lack of osteoconductive or osteoinductive factors. In this study, powder typed ß-tricalcium phosphate (ß-TCP) and hydroxyapatite (HA) were respectively blended with thermally molten PCL to use as material for 3D printed scaffold. The extrusion based 3D printer was used to fabricate blended PCL/ß-TCP and PCL/HA scaffold. In addition, the PCL/HA scaffold was coated with porcine derived collagen and then compared their efficacy of bone regeneration in rabbit radius bone critical size defects. All groups were evaluated at weeks 8 after implantation in-vivo using micro-computed tomography and histological analysis.
This study confirmed that bone is able to be regenerated using 3D printing based scaffold without seeding of cells in a rabbit model
3D cell printing system with heating unit for fabricating 3D cell-laden constructs
Kyunghyun Min, Student, Korea Polytechnic University
Artificial tissue and organ constructs fabricated by three-dimensional (3D) cell printing system is progressively gaining more attention in the fields of tissue engineering and regenerative medicine. To mimic the form and function of native tissues and organs is one of the major challenges for fabricating artificial tissue and organ constructs. Hydrogel materials such as collagen, gelatin are often a favored choice to adjust to 3D cell printing system due to not only their biocompatibility which can hold living cells but also printable property. However, these hydrogels have been problematic for their fluidic property that can be collapsed in stacking-up process and the use of toxic crosslinking reagents. To overcome these limitations, we developed 3D cell printing system with heating unit for assisting in-situ crosslinking of the printed hydrogel resulting in stable building 3D cell-laden constructs. We conformed the stability of stacking-up and cell viability of 3D cell-laden constructs fabricated by 3D cell printing system with heating unit.
|
|
