Poster Presentations
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MODULAR GLASS MICROSYSTEM FOR IMMUNO-CAPTURE OF CTCs ON PLANAR SURFACE
Jiri Smejkal, Ph.D. student, Jan Evangelista Purkyne University in Ústí nad Labem
Development of microsystems for cell-based studies is very demanding in biomedical research and has many possible applications, capturing of circulating tumor cells (CTCs) in particular [1]. In our work, the glass microsystems for receptor-based cell immobilization and shear stress testing are presented as the microsystems suitable for immuno-capture of rare CTCs.
Microscopy glass slides were used as material for microfabrication. All fabricated parts were bonded together with the biocompatible transfer contact glue.
On the substrates, different biotinylated antibodies (EpCam – specific antibody for cell capture, Anti-Mouse IgG – non-specific antibody) were immobilized. The sandwich of immobilized antibodies was built on silanized glass substrates using APTES, biotin-PEG-COOH, and streptavidin. MCF7 cell line (ATCC® HTB-22™ adenocarcinoma cell line) was used as the model CTC cell line. The blood of patient with lung adenocarcinoma was also tested for capture of real CTCs.
The presented systems were cheap to fabricate, quick and easy to assemble. Due to their construction and design, receptor-based cell immobilization and shear stress testing on different substrates was optimized with the minimal volume of antibodies. Performed tests have shown, that model CTC cell line as well as real CTCs from blood of patient with lung adenocarcinoma can be successfully captured.
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