Poster Presentations
|
New Gene Sequencing of Pullulanase from Aureobasidium pullulans
Hani Moubasher, Professor, Cairo University
Genomic DNA of pullulanase (EC 3.2.1.41), glycosyl hydrolase family 57 from Aureobasidium pullulans (AUMC 2997), was determined using polymerase chain reaction, according to Baser program, gave assembly for 16 sequenced samples from 22 samples depending on identity percent, overlap and assembly scores, different amplified fragments were sequenced as 2604 bp. and G+C content is 51.8 % with molecular mass 80 KDa. Pullulanase gene determines 8 exons, splice 1 from (312 – 246), splice 2 from (516 - 685), splice 3 from (1064 - 1161), splice 4 from (1929 - 1218), splice 5 from (1298 - 1349), splice 6 from (1932 - 2102), splice 7 from (2286 - 2173) and splice 8 from (2513 - 2422).
Pullulanase nucleotide collection from Aureobasidium pullulans was blasted which showed high similarity with S. oleracea L. and Ricinus communis using NCBI significant alignment. EMBL- EBI tools, ClustalW2 scores for pullulanase nucleotides from Aureobasidium pullulans was 54% in comparison to both S. oleracea and Zea mays.
|
|
