Poster Presentations
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Knockdown of p53 by a novel siRNA technology causes inhibition of p53-dependent DNA damage response in IMR32 neuroblastoma cell line and ß-amyloid toxicity in rat cortical neurons.
Natalie Dalecky, Tradeshow Manager, North America, Thermo Fisher Scientific
Neuroblastoma cell lines and primary neuronal cultures are commonly used as model systems for studying neuronal development, neurodegenerative diseases and cancer. Most of these cells suffer from low transfection efficiency due to the refractory nature of the cells to lipid-based transfection reagents. Therefore, the application of siRNA for inducing RNA interference (RNAi) has limited utility in these cell types.
Thermo Scientific Accell® siRNAs have a novel chemical modification pattern that enables efficient delivery of siRNA in a wide range of cell types without the need for transfection reagents. To demonstrate the utility of Accell siRNA reagents in neuronal cells, we examined their effects on the tumor suppressor, p53, which plays a pivotal role in mediating DNA damage-induced apoptosis and confers a protective effect from ß-amyloid peptide-induced neurotoxicity. .Silencing of p53 expression with Accell siRNA was found to cause inhibition of the DNA-damage response in IMR-32 neuroblastoma cells and protect primary cortical neurons from ß-amyloid toxicity.
In this study we have shown that Accell siRNA permits target validation in neuroblastoma cells as well as primary cortical neurons. The ability to modulate gene expression in these model systems demonstrates potential opportunities for functional genomic siRNA screens in the field of neuroscience.
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