Biomarker(s) for Prognosis of Brain Stroke
Rajpal Singh Kashyap,
Senior Scientist (Medical) and Head,
Central India Institute of Medical Sciences
Development of biomarker for prognosis of stroke is of urgent need for management of acute ischemic stroke (AIS) patients Our aim was to evaluate (i) time course of inflammatory cytokines in AIS patients; (ii) and to correlate inflammatory cytokine levels with Inter-Alpha Trypsin Inhibitor Heavy Chain 4 (ITIH4), and outcome after AIS. We estimated panel of twelve inflammatory cytokines and ITIH4 in serial blood sample collected at admission 24hr, 48hr, 72hr, 144hr and at the time of discharge of AIS patients (n=5). Only six cytokine were in measurable range of kit (10pg/ml) which includes IL-2, TNF a, IL10, IL-6, IL-1B, and IL-8. We found high IL-2 at admission, which decreases (P<0.05) in follow-up samples. TNF-a increases (P<0.05) initially at 24hr followed by gradual decrease (P<0.05) after 72hr. IL-10 decrease initially (P<0.05) till 72hr as compared to its level at admission and then increases (P<0.05) at after 144hrs. Similarly, ITIH4 was down regulated in early 72hrs followed by further increase with improvement of patient. ITIH4 correlate with IL-10 and CT Scan Infarct volume. Although serum level of the IL-6, IL-1B, IL-8 increases in AIS patients they did not exhibit any pattern of expression. Expression of ITIH4, IL-10, IL-2 and TNF-a can be correlated with improvement of AIS patients. Thus serial measurement of these cytokines and ITIH4 may be useful for follow-up of clinical outcome after AIS.
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