Fluorescence Polarization Immunoassay as Rapid Express Detection of Bio- and Anthropogenic Low-Molecular Toxicants in Food Products
Sergei Eremin,
Professor,
Moscow State University
Food quality control for contaminants such as veterinary drugs, pesticides, mycotoxins and many others compounds are a serious and growing problem for world. The food control must be simple, quick and cheap. Moreover, the analysis of food contaminants must be in global scale with high-throughput screening (HTS). The current methods of assays like chromatography techniques and ELISA have several limitations for HTS. Fluorescence Polarization Immunoassay (FPIA) could be the more optimal method for screening of food samples for contaminants. The FPIA is homogeneus method based on the competitive binding and the measurement of fluorescence polarization value for reaction mixture of sample (typically 10-50 µl) and immunoreagents: fluorescent-labeled antigen (tracer) and specific antibodies. The total time required for an assay is few seconds or minutes. The limit of detection for contaminants could be reached lower than typical Maximum Residue Limit (100 ng/ml or 100 ug/kg) after careful optimization antibodies and fluorescein-labeled tracers. The recent results for development of FPIAs for detection of food contaminants will be discussed.
Acknowledgements:
This investigation was financially supported by the Russian State Targeted Program “Research and Development in Priority Areas of Development of the Russian Scientific and Technological Complex for 2014–2020” (contract 14.616.21.0061 from November 11, 2015; unique identifier of applied research: RFMEFI61615X0061).
References:
David S. Smith and Sergei A. Eremin. Fluorescence polarization immunoassays and related methods for simple, high-throughput screening of small molecules. Anal. Bioanal. Chem., 391(5), 1499-1507 (2008).
Jin-Yi Yang, Yan Zhang, Hong Wang, Zhen-Lin Xu, Sergei A. Eremin, Yu-Dong Shen, Qing Wu, Hong-Tao Lei Yuan-Ming Sun. Development of fluorescence polarization immunoassay for carbofuran in food and environmental water samples. Food Agric. Immunol. 26(3), 340-355 (2015).
N.V. Beloglazova, S.A.
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