Exploring in vitro Vibrational Microspectroscopy for High Content Analysis
Hugh Byrne,
Professor,
Dublin Institute of Technology
The potential of vibrational spectroscopy, but infrared absorption and
Raman scattering, in the fields of biological and biomedical
applications has been well demonstrated. The techniques provide
molecular based profiles of the biomolecular content, in a label free,
non-destructive manner, which, in the microscopic mode, can have
subcellular resolution. Multivariate statistical analysis is commonly
employed to extract high content information concerning cellular
metabolism and response pathways. The presentation provides an over view
of efforts to advance the application of vibrational spectroscopy for
high content cellular analysis, using examples of exposure to model
nanoparticles and chemotherapeutic agents. This progress is discussed in
the light of emerging technologies in the field of spectroscopic
microscopy.
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