Screening and Quantification of Hippuric acid as a Marker of Solvent Exposure using LCMS-MS
Chowdaiah M,
PhD Scholar ,
NIMHANS
Inhalant sniffing offers fast and intense sensory experience to abusers which also contribute to its excessive use. Users can be an active user or have frequent exposure to solvents. Objective: A biochemical procedure for detection and quantification of hippuric acid as marker of solvent exposure (toluene, paint, thinner, eraser, inhalers) in urine is described and its effectiveness in clinical samples was also demonstrated. Methods: Simple chemical reaction for hippuric acid detection and quantification was improvised for clinical application, visual detection as well as quantification by LC MS-MS wavelength was viable. Current application can be applied for screen and quantification intentional as well as occupational solvent exposures. Results: Hippuric acid levels were screened among Fourteen subject with solvent use history and ten healthy volunteers were taken as control. Association between solvent contact and hippuric acid excretion was observed. Validation parameters for recovery =100%, accuracy 2.50, coefficient variance (r2), 0.998 were in acceptable range. Conclusion: Hippuric acid, a valid biomarker for solvent exposure has treatment implications also. This application is very effective in detecting solvent exposure and quantification by LC MS-MS is confirmatory tool. This cost and time effective and hold good sensitivity.
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