Cotinine Quantification among Smokers, Passive Smokers and Non Smokers using LCMS-MS
Niharika Sane,
Research Scholar,
NIMHANS
Background: This work evaluated the performance of novel rapid method to verifying smoking status among smokers, non smokers and passive smokers by measurement of cotinine, the primary metabolite of nicotine, in saliva and urine using LC-MS.
Aims and Objectives: Cotinine levels in urine and saliva were measured among active users (smoked tobacco users; n=68) and their family members to see the effect of smoking on them (passive smokers; n=17). A comparison was made with non users/non exposed group (Control group; n=33).
Material and Methods: A simple, rapid and sensitive method to quantify cotinine in saliva and urine was developed and validated. Sample preparation was minimal. The wide linear range (5-500 ng/mL) and sensitivity (limit of detection 0.8 ng/mL) allowed quantification of urine and saliva of smokers, non smokers and passive smokers.
Results: The mean cotinine levels of urine for smokers, passive smokers and non smokers were 1043.7ng/ml, 36.63ng/ml, 13.6ng/ml, while in saliva it was 327.39ng/ml, 18.31ng/ml and 9.53/ml respectively.
Conclusion: Non parametric statistical analysis using Kruskal-Wallis showed that cotinine (urine and saliva) of smokers had significantly higher levels of cotinine than passive smokers and non smokers (p<0.01). Similarly passive smokers also had significantly higher cotinine ( urine and saliva) than non-smokers (p<0.01
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