From Droplet-based Microfluidics Developments to Droplet-based Digital PCR
Valérie Taly,
CNRS Research Director, Group Leader and Professor,
Université Paris Cité
Droplet-based microfluidics has led to the development of highly
powerful systems that represent a new paradigm in High-Throughput
Screening where individual assays are compartmentalized within
microdroplet microreactors. The integration of such systems for series
of complex individual operations on droplets has offered a solution to
the necessary miniaturization and automation of individual biological
assays. By combining a decrease of assay volume and an increase of
throughput, this technology goes beyond the capacities of conventional
screening systems. We will show how by combining droplet-based
microfluidic systems and clinical advances in molecular diagnostic we
have developed an original method to perform millions of single molecule
PCR in parallel to detect and quantify a minority of mutant sequences
within a high quantity of non-mutated sequences in complex mixture of
DNA with a sensitivity and precision that was unreachable by
conventional procedures.
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