Poster Presentations
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Automated, Parallel Preparation of Novel 3D Cell Cultures Increasing throughput for a Dose Response Experiment
Cecile Villemant, Development Scientist, TAP Biosystems - Sartorius
Classical two-dimensional (2D) cell culture is used widely for biological assays, drug screening, and many more research areas. However, cells that are grown in 2D, i.e. in a monolayer, behave differently from cells in real tissues. Three-dimensional (3D) cell cultures promise to yield responses to stimuli – such as the addition of a drug candidate – that are more similar to those seen in vivo. The RAFT (Real Architecture for 3D Tissue) system allows researchers to culture cell types of their choice, in a collagen type I hydrogel with tissue-like properties. The unique feature of the RAFT process is that it increases the collagen concentration to physiological levels quickly and reproducibly while maintaining high cell viability.
We document here the automated production of four RAFT 96-well plate cell cultures in parallel on a Tecan Freedom EVO® workstation. The automation aids matrix and cellular mixing procedures and enhances reproducibility while maintaining excellent cell viability and reliability and allowing increased throughput. The cancer cell lines MCF-7 (breast cancer) and HCT-116 (colon cancer) are used as examples of typical tumoroids-forming cell lines. We present growth and viability data and compare the response from cells cultured in RAFT 3D with classical 2D to the anticancer compound doxorubicin.
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