Poster Presentations
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Lipid nanoparticles change the transcriptional footprints of lung epithelial-derived extracellular vesicles
Benyapa Tangruksa, PhD student, Högskolan i Skövde
Extracellular vesicles (EVs) transport RNA between cells. The RNA content of EVs changes depending on the environments their parental cells are in; therefore, the expression changes occur in EVs when their parental cells are treated with lipid nanoparticles (LNPs) containing mRNA encoding the vascular endothelial growth factor A (VEGF-A) protein. VEGF-A is the main dominant inducer of the growth of blood vessels. EVs were isolated before and after the delivery of LNP VEGF-A mRNA to lung epithelial cells. The total RNA was isolated from the EVs and mRNA were identified using RNA-Seq. The large-scale data was analyzed using different bioinformatics approaches. Our preliminary results indicate that the transcripts of the isolated EVs are significantly altered after the LNP treatment. We have shown that the cell's response to LNP-VEGF mRNA is evident in the transcripts of EVs. From the result, we hypothesize that parental cells might express VEGF-A after the VEGF-A mRNA-LNP delivery and activate several intracellular pathways whose transcripts also end up in secreted EVs. Several disease pathways were predicted to be activated based on the expression of the EV cargo. The study provides novel insights into which RNA molecules change in EVs after LNP treatment on the parental cells.
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