Poster Presentations
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A novel multi-organ microfluidic chip: on the way to the complexity of a living organism
Timur Samatov, Scientist, Hemule GmbH
Currently an increasing number of physiologically relevant organ-on-chip platforms are reported. Most of them are focused on modeling particular organs or their functional elements. Here we present H-chip, a novel platform capable of culturing up to six different organotypic models integrated into a single microfluidic circuit. The developed platform provides constant long-term circulation and automated replenishment of medium in the circuit. The key characteristics of the medium flow, including volumetric flow velocity and resulting shear stress, are similar to the ones found in a human organism. The medium flow-dependent increased metabolic and proliferative activities of cultured cells support the physiological relevance of the presented platform.
Controlled Deposition and Multi-layer Architecturing of Single Biomolecules using Automated Directed Capillary Assembly and Nano-contact Printing processes
Adriana Lagraulet, Field Application Specialist, Innopsys
To date, a large amount of research studies have been carried out to manipulate and arrange biomolecules at the single molecule scale using capillary forces. However, many of these techniques remain in the fundamental research field, their industrial transfer being restricted by poor repeatability and user-dependent processes.
We present an automated process for the controlled and large scale deposition of single biomolecules, relying on the use of directed capillary assembly and nano-contact printing processes. The adjustment and control of physical parameters allows for single molecule deposition, and the use of an automate operating arm ensures high reproducibility in the assembly architectures to create.
This methodology was used to assemble DNA molecules and actin filaments, evidencing two distinct assembly mechanisms. In the first one we use capillary forces to trap and elongate DNA molecules. In the second case, fluid flows created upon evaporation and local pinning of the meniscus favor F-actin polymerization.
Additionally, by sequentially aligning and printing multiple single molecule assemblies, large-scale multi-layer architectures of single molecules were also obtained.
The large-scale capabilities and reliability of our fabrication process render sophisticated single molecule biophysical measurements possible with systematic analysis over a large population for statistical relevance.
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