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SELECTBIO Conferences Advances in Separation Technology

Advances in Separation Technology Agenda



Other Track Agendas

Advances in Biodetection & Biosensors | Advances in Microarray Technology | Advances in Protein Crystallography | Advances in Separation Technology | AgriGenomics Congress | Lab-on-a-Chip European Congress | Next-Gen Sequencing Europe | Screening Europe - HCS and Assays | Screening Europe - HTS & Compound Management | World Biobanking Summit | 

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Thursday, 30 June 2011

08:00

Registration


Session: Small Molecule Applications

10:00

Micro Separation LC-MS Methods for Improving Retrospective Detection of Drug Administration for Evidential Purposes
Mark Parkin, Lecturer, Kings College London, United Kingdom

Investigating multifaceted approaches utilising micro separation LC-MS methods for improving retrospective drug detection in both regular (blood or urine) and alternative (hair, sweat or saliva) biological matrices.

10:30

Coffee Break and Networking in the Exhibition Hall

11:15

Functional Composite Phases for Biomolecule Extraction and Separations
Brett Paull, Professor, University of Tasmania, Australia

11:45

Rapid Analysis of Plants by Ambient MS and On-Chip Sample Clean-up
Teris Beek, Associate Professor, Wageningen University, Netherlands

Pros and cons of ambient MS techniques for probing plants without any sample clean-up are discussed. Additionally rapid sample clean-up of alkaloid-containing plants by miniaturised three-phase microreactors is shown.

12:15

Technology Spotlight

12:30

Lunch and Networking in the Exhibition Hall

12:45

SelectBioFree Workshop
Advancement of Separation, Purification and Crystallography from Agilent Technologies
,

13:30

SelectBioFree Workshop
High Content Screening for Compounds and Genes Affecting Bacterial and Viral Infections
,

13:30

Poster Session


Session: Protein Analysis

14:15

Arthur MoseleyKeynote Presentation

Mass Spectrometry Based Translational Proteomics – From the Benchtop to the Bedside
Arthur Moseley, Director, Duke University School of Medicine, United States of America

This presentation evaluates the experimental design factors critical for success in biomarker proteomics. Importantly, it seeks to define both the mass spectrometry and the non-mass spectrometry experimental design factors necessary for successful biomarker proteomics projects in their journey from the Benchtop to the Bedside.

14:45

Rapid and Sensitive Identification and Quantification of Fungal Cell Wall Glycoproteins
Chris de Koster, Professor, University of Amsterdam, Netherlands

In this presentation we will highlight the principles of our protein isolation, identification and quantification methods and discuss in more detail the quantitative comparison of the cell wall proteome of two growth forms of the polymorphic fungus Candida albicans, i.e., pathogenic hyphal growth vs. unicellular yeast.

15:15

Coffee Break and Networking in the Exhibition Hall

16:00

3D-Gel Electrophoresis, a New Development in Protein Analysis
Robert Ventzki, Scientist, University of Greifswald, Germany

We present a new method for automated high-throughput analysis of DNA and proteins by electrophoresis in a 3D-geometry gel with online detection of laser-induced fluorescence, allowing the rapid, simultaneous, high-resolution analysis of a multitude of samples by 1D- or 2D-separation.

16:30

Development of "Top-Down" and "Bottom-up" Approaches for the Determination of Milk and Egg Proteins in Spiked White Wine by on-line Liquid Chromatography/Tandem Mass Spectrometry
Margarita Moreno, Post Doc, European Commission, Belgium

Method development: "top-down" and "bottom-up" approaches for the determination of milk and egg proteins in spiked white wine by on-line liquid chromatography/ tandem mass spectrometry.

17:00

Remote Open Access – The Lab2Lab™ Advantage
Brian Everatt, Team Leader, Novartis, United Kingdom

This presentation will describe the system recently installed at Novartis, Horsham (UK) and demonstrate the typical throughput capability and time savings this introduces to the users, in addition to showing how it is possible to rationalize expensive equipment, reducing the cost of maintenance and support whilst increasing availability. It will show how the system can cope with failure of the analytical instrumentation by being able to redirect samples to working equipment, and the potential for future expansion.

17:30

Drinks Reception

Friday, 1 July 2011

08:00

SelectBioFree Workshop
PCR without DNA purification – Thermo Scientific Direct PCR
,


Session: Informatics for MS

09:00

We’ve Always had Data, so What’s Changed? What’s the Challenge and What’s the Solution?
John Langley, Head of Characterisation and Analytics, University of Southampton, United Kingdom

The question of whether data to knowledge is a new issue is worth considering. Is this a new phenomenon? The answer is definitely no. Is it an issue today? This time the answer is a resounding yes. This presentation considers the origins of the problem, what has changed that now brings the issue to the front. Examples will be used to show that there is a wealth of information in a variety of data from many applications, from a simple mass spectrum, tandem MS, chromatographic and molecular modelling approaches. Finally there will be an attempt to consolidate some of these approaches, together with present software solutions, into a future vision for automated Data to Information to Knowledge.


Session: Trapped Ions

09:30

Hans MaurerKeynote Presentation

Current Role of Hyphenated Low and High Resolution Mass Spectrometry in Clinical and Forensic Toxicology.
Hans Maurer, Head, Saarland University, Germany

Concepts and procedures using LC-MS techniques in the areas of toxicology and drug monitoring with special focus on multi-analyte procedures will be presented and discussed. The presentation will close with a short discussion of the potential of high resolution mass analyzers providing excellent identification power.

10:00

Field Asymmetric Waveform Ion Mobility Spectrometry Combined With Mass Spectrometry for Structural and Trace Analysis
Colin Creaser, Professor of Analytical Chemistry, Loughborough University, United Kingdom

Combining field asymmetric waveform ion mobility spectrometry with mass spectrometry provides complementary separation of gas-phase ions on the basis of differential mobility and mass-to-charge. The combination allows enhance separations of mixtures including the separation of isobaric ions.

10:30

Coffee Break and Networking in the Exhibition Hall


Session: Novel Separation Techniques and Methodologies

11:15

Comprehensive Drug Metabolite Profiling Using Multidimensional Detection Techniques
Jeroen Kool, Assistant Professor, Vrije Universiteit Amsterdam, Netherlands

The development of on-line and at-line bioaffinity screening in combination with liquid chromatography and mass spectrometry will be discussed towards metabolite profiling. Special focus on bioactivity assessment of drug metabolites and other drug-related compounds in mixtures, generated enzymatically using CYPs and BM3s, electrochemically, and/or (photo)chemically (light/ H2O2), are highlighted.

11:45

CANX - Novel Enrichment and Separation Methods for Bioanalysis
Guenther Bonn, Head, University of Innsbruck, Austria

Our research focuses on new developments in integrated sample preparation and separation systems including miniaturized solid-phase extraction (SPE), HPLC and capillary electrophoresis coupled to mass spectrometry.

12:15

AgilentTechnology Spotlight:
Ultrafast SPE-TOF Analysis Streamlines Workflow and Enables Native Detection of Complex Analytes.
Jennifer Rossi, Marketing, Agilent

Recent data will be presented that demonstrates the effectiveness of this ultrafast SPE-MS/MS system and its ability to increase laboratory efficiency without disruptions to existing laboratory workflows.

12:30

Lunch and Networking in the Exhibition Hall

12:45

SelectBioFree Workshop
Glosensor Technology: Intracellular Biosensors to Detect Signal Transduction in Living Cells
,

13:30

SelectBioFree Workshop
Automatic High Content Screening for Life Sciences
,

13:30

Poster Session

14:15

UHPLC – Raising the Pressure in the Laboratory
Tony Edge, Technical Manager, Thermofisher Scientific, United Kingdom

This presentation will initially look at some of the theory associated with this technology to get a clear understanding of why smaller particles are better for HPLC, and to investigate why there are the resulting challenges associated with this technique. From this some discussion will be made on how to convert standard HPLC methodologies to UHPLC methods, using a theoretical approach. There will also be some time spent at looking at the practical implications of running with very efficient chromatographic systems.

14:45

New Support Materials for bio HPLC
Peter Myers, Professor, University of Liverpool, United Kingdom

This paper describes two approaches that have been taken in the University of Liverpool to develop new support materials for the analysis of biomolecules by HPLC.

15:15

Coffee Break and Networking in the Exhibition Hall

16:00

Separation Performance of Modern HPLC Stationary Phases
Attila Felinger, Professor, University of Pecs, Hungary

The study of the mass transfer properties and thermodynamics of retention on modern fully porous and core-shell packing materials reveals the separation power of the latest generation of stationary phases. Results are presented focusing on the tools of stationary phase characterization.

16:30

Automation and On-line Hyphenations for Trace Analysis
Steven Wilson, Post Doc, University of Oslo, Norway

I will discuss our approaches to solving volume/solvent/time mismatches with such systems, and some solutions for more robust automation (i.e. avoid clogging when handling complex matrices).

17:00

Close of Conference


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