Next-Gen Sequencing & Advances in qPCR Agenda

Registration

CANCELLED - Genomic Landscape of DNA Repair Programs in Human Cancer
Jung Kyoon Choi, Assistant Professor, KAIST, Korea South

Phosphorylation of the histone variant y-H2AX forms H2AX that marks DNA double-strand breaks. Our findings based on the whole-genome landscape of H2AX and y-H2AX in resting and proliferating cells before and after ionizing irradiation provide insight into DNA repair programs in cancer and may have implications for cancer therapy.

The Korean Genome Project (KGP)
Jong Bhak, Director, TheragenEtex, Korea South

Korean Genome Project is a project carried out by Genome Research Foundation of Korea.

Coffee and Networking in Exhibiton Hall

Robust Analysis of Structural Variations Using Paired-end Sequencing Data
Swaine Chen, Assistant Professor, National University of Singapore, Singapore

I will present a new, robust method for paired-end sequencing data analysis to discover structural variations, using bacterial resequencing as a case study.

Next Generation Sequencing Analysis of Lung Cancer Genomes
ByungChul Kim, CEO, Clinomics Inc, United Kingdom

Several pathologically different lung cancer subtypes are analyzed by next-generation sequencing to find somatic variations. The results demonstrate that this approach is useful to detect previously known recurrent mutations as well as novel variations.

Technology Spotlight:
New Sample Technologies for Next Generation Sequencing
Peter Hahn, Senior Scientist R&D, Qiagen

QIAGEN introduces new technologies for preanalytic solutions streamlining NGS workflow.

Lunch and Networking in Exhibition Hall

Poster Viewing Session

ChIP and Methyl Sequencing, Dissecting the Subtleties of the Genome
Masoud Toloue, VP of Genomic Research, Bioo Scientific Corporation, United States of America

Strategies for massive parallel sequencing have revolutionized research across diverse scientific disciplines. Despite these advances methylated DNA immunoprecipitation, methyl-binding domain, and restriction enzyme based reduced methyl sequencing all need micrograms of genomic DNA, making them incompatible with laser capture microdissection or rare stem cell populations. We will present our latest ChIP and Methyl-Seq data on genome wide comparisons between differentiated and un-differentiated tissues and explain the significance of these patterns.

Oyster Genome Reveals Stress Adaptation and Complexity of Shell Formation
Xiaodong Fang, Bioinformatician, Leader of Plant and Animal Genome Group, Beijing Genome Institute, China

The Pacific oyster Crassostrea gigas belongs to one of the most species-rich but genomically poorly explored phyla, the Mollusca. We report the sequencing and assembly of the oyster genome using short-reads and a fosmid-pooling strategy, along with transcriptomes of development and stress response, and proteome of shell. The oyster genome is highly polymorphic and rich in repetitive sequences, with some transposable elements still actively shaping variation. Transcriptome studies reveal an extensive set of genes responding to environmental stress. Expansion of heat shock protein 70 and of inhibitors of apoptosis is likely central to the oyster’s adaptation to sessile life in the highly stressful intertidal zone. Our analyses also show shell formation in molluscs is more complex than currently understood and involves extensive participation of cells and their exosomes. The oyster genome sequence fills a void in our understanding of the Lophotrochozoa.

Coffee and Networking in Exhibiton Hall

Bacterial Transcriptome Complexity Revealed by Next-generation Sequencing
Byung-Kwan Cho, Assistant Professor, Korea Advanced Institute of Science And Technology, Korea South

Next-generation sequencing technologies are now inspiring a new understanding of the bacterial transcriptome. In this talk, it will be highlighted how this new emerging technology is now revolutionizing our understanding of the complexity, plasticity and regulation of bacterial transcriptomes. 

A Genome-wide Look at Post-Transcriptional Gene Regulation
Jason Myers, Director, Ion Torrent Systems Incorporated, United States of America

The combination of RNA Seq and RNA biochemistry is enabling a deeper understanding of the post-transcriptional regulation of gene expression.  Until recently the efficiency of next-generation sequencing has limited the number and depth of RNA Seq studies.  Ion Torrent Semiconductor Sequencing™ delivers RNA sequencing at an unprecedented speed, scalability and cost enabling in depth analysis of post-transcriptional gene regulation.

Drinks Reception

Technology Spotlight:
Application of Automated Molecular Diagnostic System for Infectious Diesases Based on Real Time PCR
Doo-Sung Cheon, Deputy Scientific Director , Korea Centers for Disease Control and Prevention


Automatic molecular diagnostic system integrated with HTS genome extraction module and real time PCR is presented for illustrating practical diagnostic application of taqman based real time PCR on several infecious diseases targets including enteric viruses, bioterrorism and viral hepatitis.

Coffee Break and Networking in the Exhibition Hall

High Throughput Mutation Detection Based on Multicolor Melting Analysis
Qingge Li, Professor, Xiamen University, China

A versatile approach is presented for multiple mutations detection based on multicolor melting analysis, which features high throughput, no post-PCR manipulations and wide compatibility with existing real-time PCR platforms. 

Technology Spotlight:
The New LightCycler® 96 System: it is so Easy to be a Lab Hero
Ralf Mauritz, Director, Roche Applied Science


The new LightCycler® 96 system is a benchtop instrument that can handle up to 96 samples using standard 96 multi well plates or 8-tube strips. A new optical module is developed that is based on 2x 96 glass fibres with 4 excitation and 4 emission filters. A white LED is used as light source and a CCD camera as detection unit. This setup supports the most common PCR formats used in the market like SYBR Green I and Hydrolysis Probes and thus enables the user to perform all common PCR applications.

Lunch and Networking in Exhibition Hall

Poster Viewing Session

Taking Expression Profiling to New Dimensions
Mikael Kubista, Professor/Founder, TATAA Biocenter AB, Sweden

Expression profiling of individual cells collected from tissue is exceeding powerful to reveal the biologically complexity. It is here used to characterize astrocyte development in the brain and also during healing after brain injury.

Coffee Break and Networking in the Exhibition Hall

Novel Solution Enables Automated qPCR Diagnostics at Point-of-Care without Loss of Accuracy
Ze'ev Russak, CTO, Azure PCR Limited, United Kingdom

Azure PCR’s technology automates analysis of qPCR data, particularly for infectious disease testing. The technology is convenient, easy to integrate with current hardware and any system, delivering rapid, accurate results for analysis of routine clinical diagnostics data.

Direct PCR of Isolated Single Cells on a Microfluidic Device for the Genomic Quantification
Izumi Kubo, Professor, Soka University, Japan

An original microfluidic device for the high-throughput isolation of single cells and its application to novel PCR/RT-PCR technique for genomic quantification of the isolated cells is described in this presentation.

Close of Conference