Advances in Genomics, Informatics and Bioanalysis Agenda

Genome-wide Analyses Reveals Novel Pancreatic Cancer Genes
Murali Bashyam, Scientist, Centre for DNA Fingerprinting and Diagnostics, India

The first part of the presentation shall highlight the use of several genome-wide approaches to identify putative important pancreatic cancer 'driver' genes, followed by a description of characterization of the 'drivers' using various approaches.

Frequency of JAK2 Exon 12 Mutations in Patients With JAK2 (V617F)-negative Myeloproliferative Disorders
Shefali Desai, Chief, iGenetic Diagnostics, India

JAK2 exon 12 mutation status is associated with erythrocytosis and atypical bone marrow morphology. Patients who test negative for JAK2V617F mutation should be tested for these mutations to classify further or rule out PV using allele-specific polymerase chain reaction (PCR) and sequencing. 

Lunch and Networking in the Exhibition Hall

Poster Viewing Session

Bioinformatics: Next-Generation Sequencing Data Analysis
Vivek Keshri, Research Associate, National Bureau of Agriculturally Important Microorganisms, India

Here, we present comparative genomics and its impact, next-generation sequencing data analysis for genome comparisons and basic approaches for the data analysis form of alignment, assembly, base calling, Chip-Seq, RNA-Seq, visualization tools and techniques.

Coffee Break and Networking in the Exhibition Hall

Characterization of a Novel Moonlighting Histone H3 Specific Protease Activity by a Known NAD+ Dependent Dehydrogenase From Chicken Liver
Jogeswar Purohit, Assistant Professor, Smt. C.H.M. College, India

Site-specific proteolysis of the N or C-terminus of histone tails has recently emerged as a novel form of irreversible post-translational modifications assigned to histones. In the present study liver specific proteolysis of histone H3 in adult chicken liver tissue is demonstrated. The proteolysis removes 23 amino acids from the N-terminus of histone H3. We further demonstrate a histone H3 specific protease (H3ase) activity in the chicken liver nuclear extract. H3ase was purified to homogeneity 

Indigenous Development of Membrane Based BioChip Cassette and Biochip Arrayer System for Making Protein Microarrays
Ratnesh Sengar, Scientific Officer, Bhabha Atomic Research Centre, India

Indigenous development of a bio-chip cassette and arrayer system for producing the antibody based bio-chip for in vitro simultaneous quantitative detection of a combination of thyroid related hormones using polycarbonate (PC) track-etched membranes (TEM) as a novel substrate.

Qualitative and Quantitative Analysis of Bioactive Alkaloides in Berberis and Mahonia Roots and use of PCA for Marker Identification for Quality Control.
Brijesh Kumar, Senior Principal Scientist, Central Drug Research Institute, India

The DART/Q-TOF/4000Qtrap LCMS techniques have been applied for the profiling of alkaloids in Berberis and Mahonia species. Chemical profiling of leaf, stem and root has been done successfully. PCA analysis has shown that DARTMS data can be used to differentiate these alkaloids containing plant species. This will help in identification of plants used for drug preparation and for the effective quality control in future.

Coffee Break & Networking in Exhibition Hall

Advances in Metabolite ID Strategies Using Hyphenated LC-MSMS Techniques
Umesh Pawa, Managing Director, AB Sciex, United Arab Emirates

A talk elaborating strategies and  workflows for simultaneous Metabolite Identification and quantitation using hyphenated LC-MS/MS technologies and novel scan modes in current day instrumentation.

Ethyl Glucuronide as a Biomarker of Alcohol Abuse
Bharat Venkatesh, Junior Scientific Officer, National Institute of Mental Health and Neurosciences, India

Sensitive and reliable analytical GC-MS method for determination of EtG in urine and serum was developed and validated. The performance of this method is sufficient to measure low EtG concentartion in both matrices. 

Lunch & Networking in Exhibition Hall

Poster Viewing Session

Coffee Break & Networking in Exhibition Hall

Immunogenicity and Pharmacokinetics Assessment of Protein Biotherapeutics: An Overview
Mallikarjun Dixit, Laboratory Director, Clinigene International Ltd, India

With the advent of Biotechnology, more and more drug products currently available in the market are biotech derived human recombinant protein biotherapeutics which have replaced the animal derived drug products. This has helped in global availability of quality drug products at a competitive price with very little or nil side effects. To utilize the growing demand for the biotechnology derived drug products, many multinational agencies have established the biopharmaceutical units in different parts of the world using Recombinant DNA technology.

Close of Conference