Microarray Technology Agenda

Registration

Coffee and Networking in Exhibition Hall

Reverse Phase Protein Arrays as a Tool to Identify New Biomarkers and Potential Therapeutic Targets for Breast Cancer Subtypes
Leanne De-Koning, Manager, Institut Curie, France

Reverse Phase Protein Arrays are a powerful tool to study protein expression and activation status in hundreds of samples. We applied this technology to study lobular and triple negative breast cancers with the aim to identify new prognostic biomarkers and therapeutic targets.

Reverse Phase Protein Array (RPPA) Applications in Translational Oncology
Ulrike Korf, Senior Scientist/Group Leader, German Cancer Research Center, Germany

New innovative, accurate, reliable and cost-effective tools are required to improve patient stratification and to bridge the gap between in vitro diagnostics and clinical decision-making. This challenge was addressed from a proteomic point-of-view, and recent data obtained by using reverse phase protein arrays (RPPA) to advance personalized medicine will be presented and discussed.

Lunch & Networking in Exhibition Hall

Functional Protein Profiling in Tissue Samples for Next Generation Molecular Diagnostics
Karl-Friedrich Becker, Head, Technical University of Munich, Germany

For functional protein profiling in clinical tissue samples it is necessary to implement tools that allow fast, precise, cheap, and simultaneous analysis of many network components while requiring only a small amount of clinical material. Reverse phase protein array (RPPA) is a promising technology that meets these requirements while enabling quantitative measurement of proteins and phosphoproteins.

Multi-platform Microarray Analysis for Interrogating FAK Function in Cancer
Bryan Serrels, Postdoctoral Research Scientist, University of Edinburgh, United Kingdom

We have recently identified a new and exciting role for FAK in orchestrating the composition and activation status of the tumour promoting microenvironment. Our strategy to delineate the function of nuclear FAK in cancer combines multiple microarray-based approaches, including gene expression arrays, RPPA, and antibody capture arrays. This will form the focus of my talk.

Coffee and Networking in Exhibition Hall

Molecular Profiling of Autoimmune Disease for Improved Diagnosis, Prognosis, and Classification
Christer Wingren, Associate Professor And Lecturer, Lund University, Sweden

We have shown that affinity proteomics can be used to profile crude, non-fractionated serum and urine proteomes, extracting molecular portraits of SLE and other autoimmune conditions, paving the way for improved (differential) diagnosis, prognosis, and classification based on a blood (urine) test, as well as for an enhanced understanding of the disease biology.

Drinks Reception

Coffee and Networking in Exhibition Hall

Skinomics: Microarrays in Dermatology and Skin Biology
Miroslav Blumenberg, Associate Professor, NYU Langone Medical Center, United States of America

Due to accessibility skin and dermatology were among the earliest objects for microarray studies. Noninvasive skin sampling targeted melanomas; additionally, carcinomas, psoriasis, wound-healing, etc. have been analyzed. Recently, using microarrays identified genes specific for epidermal stem cells and those changing during keratinization.

Isothermal Parallel Amplification of microRNAs and Microarray-based Detection
Thomas Brandstetter, Group Leader, University Freiburg, Germany

miRNAs are in the focus for biomarker validation. We present here a biochip platform for isothermal, multi-parametric miRNA amplification combined with microarray-based detection.

Lunch & Networking in Exhibition Hall

Polymer Microarrays - Discovering Polymers to Control Stem Cells
Annamaria Lilienkampf, Research and Teaching Fellow, Edinburgh University , United Kingdom

The Bradley group has developed polymer microrarrays where up to 7000 different polymer features can be inkjet synthesized on a glass slide. These thousands of substrates can be screened with stem cells in a high-throughput manner to allow the discovery of novel substrates that control and modulate stem cells. The identified polymers are rapidly scaled-up. Using this approach polymers have been identified that: (i). Bind adipose derived stem cells, controlling their differentiaiton while enabling thermal harvesting; (ii). Control and stabilize hESC derived hepatocytes with enhanced P450 functionality; and (iii). Promote bone formation from Stro+ cells.

From Microarrays to Nanoarrays: Measuring Hybridisation on Single Molecule Probes
Jens Sobek, Research Scientist, University of Zurich, Switzerland

Applying a new technology, we are trying to find new ways in order to overcome some of the limitations intrinsic to microarraying. We are studying the kinetics of molecular interactions of a single probe molecule immobilised on a chip surface. The chip consists of 160000 nanostructures with a diameter of about 100 nm. We are aiming for reducing microarray spots with respect to the number of immobilised molecules from about 10^12 to 1, and performing experiments with a single molecule per nanostructure.

Coffee and Networking in Exhibition Hall

Carbohydrate Arrays and Glyconanoparticles: Immunity, Infection and Bioterrorism
Rob Field, Project Leader, John Innes Centre, United Kingdom

Close of Conference