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SELECTBIO Conferences Circulating Biomarkers 2021

Circulating Biomarkers 2021 Agenda


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Thursday, 25 February 2021

00:00

Michael GranerConference Chair

Title to be Confirmed.
Michael Graner, Associate Professor, Dept of Neurosurgery, University of Colorado Anschutz School of Medicine, United States of America

00:00

Lucia LanguinoConference Chair

Title to be Confirmed.
Lucia Languino, Professor of Cancer Biology, Thomas Jefferson University, United States of America

00:00

Steve SoperKeynote Presentation

Repurposing Microfluidic Tools for the Analysis of Liquid Biopsy Markers for COVID-19 Diagnostics
Steve Soper, Foundation Distinguished Professor, Director, Center of BioModular Multi-scale System for Precision Medicine, The University of Kansas, Adjunct Professor, Ulsan National Institute of Science & Technology, United States of America

Extracellular vesicles (EVs), which are sub-micrometer sized particles produced from biological cells, can be isolated from liquid biopsy samples and used to manage a variety of diseases including the oncology-related diseases as well as others. However, disease-associated EVs must first be isolated in high purity to secure quality molecular data because even non-diseased cells produce EVs that can mask subtle disease-related molecular alterations. We have developed a plastic microfluidic chip, which is injection molded, consisting of 1.5M micropillars decorated with affinity agents to allow for selecting EVs from plasma. We have repurposed this chip to select SARS-CoV-2 virus particles (VPs) directly from saliva. In this presentation, we discuss an assay that can accept saliva samples and search for VPs (VP selection chip) and then, count the number of VPs selected using a label-free approach; nano-Coulter Counter chip (nCC). The VP selection chip affinity targeted SARS-CoV-2 VPs using an aptamer surface immobilized to pillars contained within the chip that were directed against the SARS-CoV-2 Spike protein. The VP selection chip allowed for affinity loading up to 1010 SARS-CoV-2 VPs. Following selection (VP recovery >40%) and subsequent release (~89% efficiency) from the VP selection chip using a photocleavable linker and a blue-light LED, the selected particles were counted using the nCC chip. For high throughput VP counting, 5 nCCs were placed in parallel and offered 100% detection efficiency for VPs travelling through a 200 nm in-plane pore. The entire assay could be completed in ~20 min with full process automation. In addition, the selected particles were available for analysis of their RNA content using RT-qPCR. For a 20 sample set, RT-qPCR identified 4 samples of infected individuals and our assay was successful in identifying all 4 of those samples. The chips and controlling electronics could be packaged into a handheld instrument to allow for point-of-care testing or even at-home testing.

00:00

Utkan DemirciKeynote Presentation

Title to be Confirmed.
Utkan Demirci, Professor, Stanford University School of Medicine, United States of America

00:00

Jennifer JonesKeynote Presentation

Title to be Confirmed.
Jennifer Jones, NIH Stadtman Investigator, Head of Transnational Nanobiology, Laboratory of Pathology, Center for Cancer Research, National Cancer Institute, United States of America

00:00

Liquid Biopsy -- Exosomal microRNA Biomarker Signatures in Various Stages of Pneumonia in Covid-19 Patients
Michael Pfaffl, Professor, Technical University of Munich, Germany


Agenda is not currently available
Add to Calendar ▼2021-02-25 00:00:002021-02-26 00:00:00Europe/LondonCirculating Biomarkers 2021Circulating Biomarkers 2021 in San Diego, CaliforniaSan Diego, CaliforniaSELECTBIOenquiries@selectbiosciences.com