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SELECTBIO Conferences Single Cell Analysis Summit

Single Cell Analysis Summit Agenda



Co-Located Conference Agendas

Lab-on-a-Chip World Congress | Microarray World Congress | Molecular Diagnostics World Congress | Single Cell Analysis Summit | 

Print Agenda

Tuesday, 25 September 2012

08:00

Registration


Microfluidics & Single Cell Analysis

09:00

High Throughput Microfluidic Single Cell Digital PCR
Kevin Heyries, Researcher, University of British Columbia, Canada

We have developed an integrated high throughput microfluidic device for the analysis of 200 single cells per run using digital PCR. We demonstrate the absolute quantification of mRNA transcripts (GAPDH and BCR-ABL) as well as microRNA (miR16) with single cell resolution.


Detection, Isolation and Analysis of CTCs/Single Cells

09:30

Quantitation of Reactive Oxygen Species and Oxidative Damage in Single Mammalian Cells.
Edgar Arriaga, Professor, University of Minnesota, United States of America

This presentation describes key features of capillary electrophoresis for the analysis of single cells of various shapes. It focuses on the selection and application of molecular probes to target reactive oxygen species and markers of molecular damage.

10:00

NanoVelcro-Embedded Microchips for Detection and Isolation of Circulating Tumor Cells: Validation Studies in Oncology Clinic
Hsian-Rong Tseng, Professor, Crump Institute for Molecular Imaging, California NanoSystems Institute, University of California-Los Angeles, United States of America

This presentation will introduce a new type of cell-affinity assay that is capable of detecting circulating tumor cells (CTCs) in blood samples collected from metastatic cancer patients.  Similar to most of the existing approaches, anti-EpCAM was grafted onto the surfaces to distinguish CTCs from the surrounding hematologic cells.  The uniqueness of our technology is the use of nanostructured surfaces, which facilitates local topographical interactionsbetween CTCs and substrates at the very first cell/substrate contacting time point. We demonstrated the ability of these nanostructured substrates to capture CTCs in whole blood samples with significantly improved efficiency and selectivity.

10:30

Coffee Break and Networking in the Exhibition Hall


Tools & Technologies for Single Cell Analysis

11:15

Steven BoxerKeynote Presentation

Membrane Fusion and Imaging in Systems of Increasing Complexity - From Single Vesicles to Single Brain Cells
Steven Boxer, Professor, Stanford University, United States of America

Our lab has developed model membrane systems to mimic individual cell membranes, both to study fundamental features and as tools in biotechnology.  I will focus on new methods to manipulate these membranes that can be used to target single cells. 

11:45

Flexible Patterning of the Cellular Microenvironment for Single Cell Analysis
John Collins, Senior Applications Scientist, NanoInk Inc, United States of America

Tip based lithography is a unique and powerful way to fabricate complex scaffolds for investigating cell behavior at single cell levels.  Multiplexed deposition of protein and biocompatible polymer features at subcellular sizes enables the fabrication of single cell microenvironmental arrays.  

12:15

Lunch and Networking in the Exhibition Hall

13:30

Poster Viewing Session

14:15

Quantitative Analysis of Intercellular Interactions Using Supported Membrane Interfaces
Nina Caculitan, Researcher, University of California San Diego, United States of America

Supported membranes interfaced with a T cell as a paradigmatic model yield high information content data of cell behavior during an immune response. This adaptable approach is extendable to other systems to study disease pathology for a cell population.

14:45

Detection of Rare Cancer Cell Phenotypes by Single Cell Analysis of Surface Proteins
Rajan Kumar, President, Genome Data Systems, Inc., United States of America

Cells with aggressive and metastatic potential are rare and their phenotypes are overwhelmed by more abundant cells. Presence of rare cell phenotypes and differential protein expression in breast and pancreatic cancer cell lines, and in primary and metastatic mouse xenografts were determined using a novel single cell analysis method.

15:15

Coffee Break and Networking in the Exhibition Hall


Single Cell Analysis in Signalling

16:00

Imaging Molecular Dynamics in Single Embodied Cells
Thai Truong, Senior Researcher, California Institute of Technology, United States of America

16:30

Quantification Noise in Single Cell Experiments
Michael Pfaffl, Professor, Technical University of Munich, Germany

In quantitative single-cell studies, the critical part is the low amount of nucleic acids present and the resulting experimental variations. In addition biological data obtained from heterogeneous tissue are not reflecting the expression behaviour of every single-cell. These variations can be derived from natural biological variance or can be introduced externally. Both have negative effects on the quantification result. The aim of this study is to make quantitative single-cell studies more transparent and reliable in order to fulfil the MIQE guidelines at the single-cell level.


Single Cell Genomics/Transcriptomics

17:00

Norman DovichiKeynote Presentation

An Approach to Single Cell Protein Analysis
Norman Dovichi, Professor, University of Notre Dame, United States of America

Capillary electrophoresis coupled with laser-induced fluorescence has allowed study of proteins from single cells. More recently, the use of high sensitivity mass spectrometers allows the identification of proteins from minute samples, which should have value in single cell analysis.

17:30

Drinks Reception

Wednesday, 26 September 2012


Single Cell Genomics/Transcriptomics Cont'd

09:30

James EberwineKeynote Presentation

RNA memory and the Sentinel RNA Hypothesis
James Eberwine, Professor, University of Pennsylvania, United States of America

The cellular RNA expression profile embodies the phenotypic memory of a cell.  This is enhanced by appropriate cytoplasmic RNA splicing.

10:00

Gene Expression Analysis for Single Cells
Masataka Shirai, Senior Researcher, Hitachi, Japan

For analyzing gene expressions in single-cells, qPCR with a reusable single-cell cDNA library method, none-bias cDNA amplification coupled with a next generation DNA sequencer, and a new classification method for single cell gene expressions were developed. They have been successfully used for analyzing mesenchymal cells. 

10:30

Coffee Break and Networking in the Exhibition Hall

11:15

Single Cell Approach to Defining Osteoblast Cell Populations from Cortical Bone
James Flynn, Research Associate, Buck Institute for Research on Aging, United States of America

A study using gene expression profiling of single cells from bone matrix to more clearly define the osteoblast from other cell populations using lineage specific markers. This approach has potential applications where precursor cells must be delineated from differentiated cells.  

11:45

Taking Cancer Genomics to the Single Cell Level
James Hicks, Research Professor, Cold Spring Harbor Laboratory, United States of America

Single cell genome and transcriptome analysis has the power to provide new insights into tumor evolution, metastasis and response to therapy in both model systems and clinical settings. Application of this technology to tumor biology and minimally invasive methods for cancer monitoring will be discussed. 

12:15

Lunch and Networking in the Exhibition Hall

13:30

Poster Viewing Session

14:15

Single-Cell Exome Sequencing Characterizes the Single-Nucleotide Mutations and Tumor evolution of Blood and Kidney Tumors
Luting Song, Bioinformatist, BGI Shenzhen, China

With devising a kind of advanced whole-genome single-cell sequencing, we use single-cell exome sequencing to characterize the single-nucleotide mutations and tumor evolution of two tumors: a JAK2-negative myeloproliferative neoplasm and a clear cell renal cell carcinoma.

14:45

Single Cell Analysis of Transcriptional Heterogeneity in the Early Developing Kidney
Steve Potter, Professor, Cincinnati Children's Hospital Medical Center, United States of America

We study the heterogeneity present among histologically homogeneous cells in early development using single cell global transcriptional profiling. Surprising bipotential cells are identified in the kidney metanephric mesenchyme cloud, and pioneer neural crest cells are examined during craniofacial development.

15:15

Coffee Break and Networking in the Exhibition Hall

15:45

Heterogeneity of Metabolic and Gene Expression Responses to Hypoxia in Individual Pre-Malignant Esophageal Epithelial Cells
Laimonas Kelbauskas, Assistant Research Professor, Arizona State University, United States of America

We present a study on the heterogeneity of metabolic and gene transcription responses to hypoxia in individual esophageal epithelial cells derived from differing stages of pre-malignant progression. We utilize a custom experimental platform for multiparameter characterization of cellular metabolic profiles.

16:15

Kinase Signaling in Single Cancer Cells
Nancy Allbritton, Professor and Chair, University of North Carolina, United States of America

High-sensitivity, chemical separations to characterize the contents of single cells is emerging as an important approach to identify signaling pathways in tumor cells and automated microengineered platforms for these assays provide the experimentalist with unprecedented opportunities to understand cell behavior. 

16:45

Close of Conference


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